BRENDA - Enzyme Database

Characterization of ornithine carbamoyltransferase from cultured carrot cells of low embryogenic potential

Baker, S.R.; Yon, R.J.; Phytochemistry 22, 2171-2174 (1983)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.3.3
1,4-diaminobutane
100 mM, 50% inhibition
Daucus carota
2.1.3.3
agmatine
13 mM, 50% inhibition
Daucus carota
2.1.3.3
arginine
L-isomer, 8 mM, 50% inhibition
Daucus carota
2.1.3.3
L-norvaline
2 mM, 50% inhibition
Daucus carota
2.1.3.3
additional information
not inhibited by lysine and alpha-methyl-L-ornithine
Daucus carota
2.1.3.3
phosphate
competitive vs. carbamoylphosphate
Daucus carota
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.3.3
158000
-
gel filtration
Daucus carota
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.3.3
Daucus carota
-
carrot
-
Purification (Commentary)
EC Number
Commentary
Organism
2.1.3.3
-
Daucus carota
Reaction
EC Number
Reaction
Commentary
Organism
2.1.3.3
carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
ordered sequential mechanism, carbamoylphosphate binds first, followed by L-ornithine, L-citrulline leaves first, followed by phosphate
Daucus carota
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.3.3
cell suspension culture
-
Daucus carota
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.3.3
carbamoyl phosphate + L-ornithine
-
485916
Daucus carota
phosphate + L-citrulline
-
485916
Daucus carota
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.3.3
7.5
-
half-maximal activity at pH 9.0
Daucus carota
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.3.3
7.5
9
approx. 50% of maximal activity at pH 9.0
Daucus carota
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.1.3.3
1,4-diaminobutane
100 mM, 50% inhibition
Daucus carota
2.1.3.3
agmatine
13 mM, 50% inhibition
Daucus carota
2.1.3.3
arginine
L-isomer, 8 mM, 50% inhibition
Daucus carota
2.1.3.3
L-norvaline
2 mM, 50% inhibition
Daucus carota
2.1.3.3
additional information
not inhibited by lysine and alpha-methyl-L-ornithine
Daucus carota
2.1.3.3
phosphate
competitive vs. carbamoylphosphate
Daucus carota
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.3.3
158000
-
gel filtration
Daucus carota
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.3.3
-
Daucus carota
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.1.3.3
cell suspension culture
-
Daucus carota
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.3.3
carbamoyl phosphate + L-ornithine
-
485916
Daucus carota
phosphate + L-citrulline
-
485916
Daucus carota
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.1.3.3
7.5
-
half-maximal activity at pH 9.0
Daucus carota
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.1.3.3
7.5
9
approx. 50% of maximal activity at pH 9.0
Daucus carota