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Literature summary extracted from

  • Baker, S.R.; Yon, R.J.
    Characterization of ornithine carbamoyltransferase from cultured carrot cells of low embryogenic potential (1983), Phytochemistry, 22, 2171-2174.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 1,4-diaminobutane 100 mM, 50% inhibition Daucus carota
2.1.3.3 agmatine 13 mM, 50% inhibition Daucus carota
2.1.3.3 arginine L-isomer, 8 mM, 50% inhibition Daucus carota
2.1.3.3 L-norvaline 2 mM, 50% inhibition Daucus carota
2.1.3.3 additional information not inhibited by lysine and alpha-methyl-L-ornithine Daucus carota
2.1.3.3 phosphate competitive vs. carbamoylphosphate Daucus carota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 158000
-
 gel filtration Daucus carota

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Daucus carota
-
 carrot
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3
-
 Daucus carota

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.3.3 carbamoyl phosphate + L-ornithine = phosphate + L-citrulline ordered sequential mechanism, carbamoylphosphate binds first, followed by L-ornithine, L-citrulline leaves first, followed by phosphate Daucus carota
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.3 cell suspension culture
-
 Daucus carota
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Daucus carota phosphate + L-citrulline
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 7.5
-
 half-maximal activity at pH 9.0 Daucus carota

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.3.3 7.5 9 approx. 50% of maximal activity at pH 9.0 Daucus carota