EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.3.2 | P268A | 40fold reduction in activity, concentration of N-(phosphonoacetyl)-L-aspartate for maximal activation is increased 233fold as compared to the wild-type, less activation by ATP, stronger inhibition by CTP | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.3.2 | additional information | - |
additional information | sigmoidal saturation curve for aspartate | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.3.2 | Zn2+ | - |
Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.2 | carbamoylphosphate + L-aspartate | Escherichia coli | - |
phosphate + N-carbamoyl-L-aspartate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.3.2 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.3.2 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.3.2 | carbamoyl phosphate + L-aspartate | - |
Escherichia coli | phosphate + N-carbamoyl-L-aspartate | - |
? | |
2.1.3.2 | carbamoylphosphate + L-aspartate | - |
Escherichia coli | phosphate + N-carbamoyl-L-aspartate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.3.2 | More | holoenzyme is a dodecamer composed of 2 catalytic trimers and 3 regulatory dimers, the catalytic chain is composed of 2 structural domains, the aspartate domain and the carbamoylphosphate domain which are involved in the binding of aspartate and carbamoylphosphate respectively, the regulatory chain is also composed of 2 domains, the allosteric and the zinc domains, which are involved in the binding of allosteric effectors and zinc | Escherichia coli |