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Literature summary extracted from

  • Achar, B.S.; Savithri, H.S.; Vaidyanathan, C.S.; Rao, N.A.
    Studies on plant aspartate transcarbamylase. Purification and properties of the enzyme from mung-bean (Phaseolus aureus) seedlings (1974), Eur. J. Biochem., 47, 15-22.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.3.2 purified enzyme is inactivated if stored frozen Vigna radiata var. radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.2 5'-UMP 2'-UMP and 3'-UMP have no effect Vigna radiata var. radiata
2.1.3.2 ATP 1 mM, 40% inhibition Vigna radiata var. radiata
2.1.3.2 GTP 1 mM, 20% inhibition Vigna radiata var. radiata
2.1.3.2 Maleate
-
 Vigna radiata var. radiata
2.1.3.2 succinate
-
 Vigna radiata var. radiata
2.1.3.2 UDP 1 mM, 45% inhibition Vigna radiata var. radiata
2.1.3.2 UMP 1 mM, 75% inhibition Vigna radiata var. radiata
2.1.3.2 UTP 1 mM, 40% inhibition Vigna radiata var. radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 128000
-
 gel filtration, sedimentation data Vigna radiata var. radiata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Vigna radiata var. radiata
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Vigna radiata var. radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 MnSO4, ammonium sulfate, DEAE-cellulose, Sephadex G-100 Vigna radiata var. radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.2 seedling
-
 Vigna radiata var. radiata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.2 2.7
-
-
 Vigna radiata var. radiata

Storage Stability

EC Number Storage Stability Organism
2.1.3.2 0-4°C, 1-2 days Vigna radiata var. radiata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate specific for L-aspartate Vigna radiata var. radiata phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Vigna radiata var. radiata phosphate + N-carbamoyl-L-aspartate
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.3.2 30 75 approx. 70% of maximal activity at 30°C, approx. 30% of maximal activity at 75°C Vigna radiata var. radiata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.2 50
-
 5 min, no loss of activity Vigna radiata var. radiata
2.1.3.2 55
-
 5 min, 20% loss of activity Vigna radiata var. radiata
2.1.3.2 60
-
 5 min, 50% loss of activity Vigna radiata var. radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.2 8
-
 maxima at pH 8.0 and pH 10.2 Vigna radiata var. radiata
2.1.3.2 10.2
-
 maxima at pH 8.0 and pH 10.2 Vigna radiata var. radiata