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Literature summary extracted from

  • Chang, T.Y.; Jones, M.E.
    Aspartate transcarbamylase from Streptococcus faecalis. Purification, properties, and nature of an allosteric activator site (1974), Biochemistry, 13, 629-638.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.3.2 4-aminobutyrate
-
 Enterococcus faecalis
2.1.3.2 Aminoethanol activates Enterococcus faecalis
2.1.3.2 Butyrate
-
 Enterococcus faecalis
2.1.3.2 Carbamoyl-beta-alanine
-
 Enterococcus faecalis
2.1.3.2 formate
-
 Enterococcus faecalis
2.1.3.2 Hydroxylamine-HCl 30 mM, 2120% activation Enterococcus faecalis
2.1.3.2 Imidazole-HCl 30 mM, 2710% activation Enterococcus faecalis
2.1.3.2 Isobutyrate
-
 Enterococcus faecalis
2.1.3.2 L-2-aminobutyrate
-
 Enterococcus faecalis
2.1.3.2 additional information at high aspartate levels addition of an activator has no effect Enterococcus faecalis
2.1.3.2 NaCl 30 mM, 1730% activation Enterococcus faecalis
2.1.3.2 NH4Cl 30 mM, 2710% activation Enterococcus faecalis
2.1.3.2 propionate
-
 Enterococcus faecalis
2.1.3.2 Triethanolamine-HCl 30 mM, 1950% activation Enterococcus faecalis
2.1.3.2 Tris-HCl 30 mM, 2620% activation Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 128000
-
 sedimentation equilibrium Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Enterococcus faecalis
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Enterococcus faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2
-
 Enterococcus faecalis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.1.3.2 4% of original activity is restored Enterococcus faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.2 350
-
-
 Enterococcus faecalis

Storage Stability

EC Number Storage Stability Organism
2.1.3.2 -20°C, 0.1-1 mg protein/ml, at least 2 years, no loss of activity 4°C, 0.1-1 mg/ml, at least 2 months, no loss of activity Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Enterococcus faecalis phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Enterococcus faecalis phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 tetramer
-
 Enterococcus faecalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.3.2 6.6
-
-
 Enterococcus faecalis