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Literature summary extracted from

  • Brabson, J.S.; Maurizi, M.R.; Switzer, R.L.
    Aspartate transcarbamylase from Bacillus subtilis (1985), Methods Enzymol., 113, 627-635.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.3.2 50 mM Tis-HCl, pH 8.1, 70% ammonium sulfate Bacillus subtilis

General Stability

EC Number General Stability Organism
2.1.3.2 carbamoyl phosphate protects against proteolysis Bacillus subtilis
2.1.3.2 carboxylic acids, e.g. acetic acid, citric acid and high ionic strength, 200 mM KCl, protect against thermal denaturation at 65-70°C Bacillus subtilis
2.1.3.2 unstable in very dilute solutions Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.2 N-(Phosphonoacetyl)-L-aspartate
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.2 0.11
-
 Carbamoyl phosphate
-
 Bacillus subtilis
2.1.3.2 7
-
 L-aspartate
-
 Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Bacillus subtilis
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 protamine sulfate, heat treatment, ammonium sulfate, Sephadex G-150, DEAE-Sephadex, DEAE-cellulose Bacillus subtilis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.3.2 carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate reaction mechanism Bacillus subtilis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.2 560
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
2.1.3.2 -10°C -20°C, many months, no loss of activity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Bacillus subtilis phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Bacillus subtilis phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 trimer
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.2 additional information
-
 carboxylic acids e.g. acetic acid, citric acid and high ionic strength, 200 mM KCl, protect against thermal denaturation at 65-70°C Bacillus subtilis
2.1.3.2
-
 25 quite stable Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.2 8.5
-
-
 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.3.2 6
-
 unstable below Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.2 0.000006
-
 N-(Phosphonoacetyl)-L-aspartate
-
 Bacillus subtilis