Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Swyryd, E.A.; Seaver, S.S.; Stark, G.R.
    N-(phosphonacetyl)-L-aspartate, a potent transition state analog inhibitor of aspartate transcarbamylase, blocks proliferation of mammalian cells in culture (1974), J. Biol. Chem., 249, 6945-6950.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.2 4,5-dicarboxy-2-ketopentylphosphonate
-
 Mesocricetus auratus
2.1.3.2 N-(phosphonacetyl)-L-aspartate
-
 Mesocricetus auratus
2.1.3.2 N-(Phosphonoacetyl)-L-aspartate
-
 Escherichia coli
2.1.3.2 N-(Phosphonoacetyl)-L-aspartate
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.2 0.004
-
 Carbamoyl phosphate in crude extracts of fibroblasts Mesocricetus auratus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Mus musculus
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Escherichia coli
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate Mesocricetus auratus
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Escherichia coli
-
-
-
2.1.3.2 Mesocricetus auratus
-
-
-
2.1.3.2 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.2 cell culture fibroblast cell line MA-134 Mus musculus
-
2.1.3.2 cell culture simian virus 40 transformed syrian hamster cell line Mesocricetus auratus
-
2.1.3.2 spleen
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Mus musculus phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Mesocricetus auratus phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Mus musculus phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
 ?
2.1.3.2 carbamoylphosphate + L-aspartate
-
 Mesocricetus auratus phosphate + N-carbamoyl-L-aspartate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.2 0.000001
-
 N-(phosphonacetyl)-L-aspartate
-
 Mesocricetus auratus
2.1.3.2 0.00001
-
 4,5-dicarboxy-2-ketopentylphosphonate
-
 Mesocricetus auratus