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Literature summary extracted from

  • Sheperdson, M.; Pardee, A.B.
    Production and crystallization of aspartate transcarbamylase (1960), J. Biol. Chem., 235, 3233-3237.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.3.2 approx. 30% ammonium sulfate, crystals appear after about 2 weeks at 4°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.2 220000
-
analytical ultracentrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoylphosphate + L-aspartate Escherichia coli catalyzes the formation of carbamoyl-L-aspartate, the first compound unique to the biosynthetic pathway for pyrimidine nucleotides phosphate + N-carbamoyl-L-aspartate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 heat, ammonium sulfate, DEAE-cellulose Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.3.2 116.7
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.3.2 4°C, mercaptoethanol-imidazole buffer, several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
?
2.1.3.2 carbamoylphosphate + L-aspartate catalyzes the formation of carbamoyl-L-aspartate, the first compound unique to the biosynthetic pathway for pyrimidine nucleotides Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.3.2 417
-
L-aspartate
-
Escherichia coli