Literature summary extracted from

  • Vial, L.; Gomez, P.; Panvert, M.; Schmitt, E.; Blanquet, S.; Mechulam, Y.
    Mitochondrial methionyl-tRNAfMet formyltransferase from Saccharomyces cerevisiae: Gene disruption and tRNA substrate specificity (2003), Biochemistry, 42, 932-939.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.9 cloning and expression of the gene encoding mitochondrial enzyme in Escherichia coli Pal13Tr-pMAF and in yeast, several strains lacking gene exhibit in minimal medium a thermosensitive respiratory growth and a slower growth rate Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 additional information
-
additional information kinetic parameters of the formylation of various mutant methionyl-tRNAs Saccharomyces cerevisiae
2.1.2.9 0.0005
-
E. coli L-valyl-tRNAfMet
-
Saccharomyces cerevisiae
2.1.2.9 0.0003
-
E. coli L-methionyl-tRNAfMet
-
Saccharomyces cerevisiae
2.1.2.9 0.0003
-
E. coli L-isoleucyl-tRNAfMet
-
Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.9 mitochondrion
-
Saccharomyces cerevisiae
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Saccharomyces cerevisiae initiation of protein synthesis in mitochondria tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Saccharomyces cerevisiae
-
strains CMY214, W303, YPALS, DBY2057
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.9 purification of recombinant enzyme, expressed in Escherichia coli Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure