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Literature summary extracted from

  • Gite, S.; Li, Y.; Ramesh, V.; RajBhandary, U.L.
    Escherichia coli methionyl-tRNA formyltransferase: Role of amino acids conserved in the linker region and in the C-terminal domain on the specific recognition of the initiator tRNA (2000), Biochemistry, 39, 2218-2226.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.9 gene encoding MTF is cloned and sequenced, expression of wild-type and mutant enzymes in Escherichia coli Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.9 crystal structure of enzyme complexed with formylmethionyl-tRNAfMet Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.9 additional information several C-terminal deletion mutants and substitution mutants in the linker region between the C- and N-terminal domain and the C-terminal domain Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 additional information
-
 additional information values for several mutant enzymes in the linker region and C-terminal domain Escherichia coli
2.1.2.9 0.0005
-
 E. coli L-methionyl-tRNAfMet
-
 Escherichia coli
2.1.2.9 0.0037
-
 L-methionyl-tRNAfMet MTFDeltaC20 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Escherichia coli P23882
-
-
2.1.2.9 Escherichia coli JM109 P23882
-
-
2.1.2.9 Escherichia coli XL1-Blue P23882
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.9 purification of His-tagged wild-type and C-terminal deletion mutant recombinant enzymes, expressed in Escherichia coli Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.9 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + E. coli L-methionyl-tRNAfMet
-
 Escherichia coli tetrahydrofolate + E. coli N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O role of the linker region between N- and C-terminal domain Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity determinants at the end of the acceptor stem of tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity is restricted to methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O recognition mechanism of methionyl-tRNAfMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O important role of a 16-amino acid insertion loop in enzyme for tRNA recognition Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O role of the linker region between N- and C-terminal domain Escherichia coli JM109 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity determinants at the end of the acceptor stem of tRNAfMet Escherichia coli JM109 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site Escherichia coli JM109 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O role of the linker region between N- and C-terminal domain Escherichia coli XL1-Blue tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O specificity determinants at the end of the acceptor stem of tRNAfMet Escherichia coli XL1-Blue tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O C-terminal region is very important for enzyme activity: basic amino acids contribute mostly toward the nonspecific binding and orientation of the tRNA 3'-end toward the catalytic site Escherichia coli XL1-Blue tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 additional information no formylation of methionyl-tRNAmMet Escherichia coli ?
-
 ?
2.1.2.9 additional information enzyme structure Escherichia coli ?
-
 ?
2.1.2.9 additional information no formylation of methionyl-tRNAmMet Escherichia coli JM109 ?
-
 ?
2.1.2.9 additional information enzyme structure Escherichia coli JM109 ?
-
 ?
2.1.2.9 additional information no formylation of methionyl-tRNAmMet Escherichia coli XL1-Blue ?
-
 ?
2.1.2.9 additional information enzyme structure Escherichia coli XL1-Blue ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.9 additional information
-
 additional information values for several mutant enzymes in the linker region and C-terminal domain Escherichia coli
2.1.2.9 0.2
-
 L-methionyl-tRNAfMet MTFDeltaC20 Escherichia coli
2.1.2.9 37.3
-
 L-methionyl-tRNAfMet wild-type enzyme Escherichia coli