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Literature summary extracted from

  • Leis, J.P.; Keller, E.B.
    N-formylmethionyl-tRNA of wheat chloroplasts. Its synthesis by a wheat transformylase (1971), Biochemistry, 10, 889-894.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.9 chloroplast
-
 Triticum aestivum 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.9 K+ activates, maximum activity at 5 mM Mg2+ and 20 mM KCl Triticum aestivum
2.1.2.9 Mg2+ requirement Triticum aestivum
2.1.2.9 Mg2+ maximum activity at 5 mM Mg2+ and 20 mM KCl Triticum aestivum
2.1.2.9 Na+ activates, can replace K+ Triticum aestivum
2.1.2.9 NH4+ activates, can replace K+ Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.9 45000
-
 gel filtration Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Triticum aestivum component of a protein chain initiating system in chloroplasts tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Triticum aestivum
-
 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.9 partial Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.9 germ
-
 Triticum aestivum
-
2.1.2.9 leaf higher specific activity in isolated chloroplasts than in the remainder of the leaf extract Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.9 additional information
-
-
 Triticum aestivum

Storage Stability

EC Number Storage Stability Organism
2.1.2.9 -20°C, longer than 4 months, stable Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from wheat germ and leaf Triticum aestivum tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Triticum aestivum tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O component of a protein chain initiating system in chloroplasts Triticum aestivum tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 additional information no formylation of cytoplasmic initiating Met-tRNAi Triticum aestivum ?
-
 ?
2.1.2.9 additional information no formylation of methionyl-tRNAmMet Triticum aestivum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.9 37
-
 assay at Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.9 7.5
-
-
 Triticum aestivum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.9 6.5 8 good activity between Triticum aestivum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.9 10-formyltetrahydrofolate
-
 Triticum aestivum
2.1.2.9 tetrahydrofolate
-
 Triticum aestivum