EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.9 | tRNAfMet | non-aminoacylated tRNAfMet is a competitive inhibitor | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.9 | 0.0006 | - |
L-methionyl-tRNAfMet | - |
Escherichia coli | |
2.1.2.9 | 0.0013 | - |
phenylalanyl-tRNAfMet | - |
Escherichia coli | |
2.1.2.9 | 0.0014 | - |
valyl-tRNAfMet | - |
Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.1.2.9 | cytoplasm | - |
Escherichia coli | 5737 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | Escherichia coli K12 MO | involved in initiation of protein biosynthesis | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.9 | Escherichia coli | - |
lot 15-290 | - |
2.1.2.9 | Escherichia coli K12 MO | - |
lot 15-290 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet | - |
Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
r | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from E. coli | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specific for methionyl-tRNAfMet, specificity depends exclusively on the nature of tRNA moiety of aminoacylated tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | formylates methionyl-tRNAfMet from E. coli | Escherichia coli K12 MO | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | specific for methionyl-tRNAfMet, specificity depends exclusively on the nature of tRNA moiety of aminoacylated tRNAfMet | Escherichia coli K12 MO | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O | involved in initiation of protein biosynthesis | Escherichia coli K12 MO | tetrahydrofolate + N-formylmethionyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O | mischarged E. coli tRNAfMet, reduced velocity compared to the formylation of methionyl-tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylphenylalanyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + phenylalanyl-tRNAfMet + H2O | mischarged E. coli tRNAfMet, reduced velocity compared to the formylation of methionyl-tRNAfMet | Escherichia coli K12 MO | tetrahydrofolate + N-formylphenylalanyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + valyl-tRNAfMet + H2O | mischarged E. coli tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylvalyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + valyl-tRNAfMet + H2O | reduced velocity compared to the formylation of methionyl-tRNAfMet | Escherichia coli | tetrahydrofolate + N-formylvalyl-tRNAfMet | - |
? | |
2.1.2.9 | 10-formyltetrahydrofolate + valyl-tRNAfMet + H2O | mischarged E. coli tRNAfMet | Escherichia coli K12 MO | tetrahydrofolate + N-formylvalyl-tRNAfMet | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.9 | 37 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.9 | 10-formyltetrahydrofolate | - |
Escherichia coli | |
2.1.2.9 | tetrahydrofolate | - |
Escherichia coli |