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Literature summary extracted from

  • Blanquet, S.; Dessen, P.; Kahn, D.
    Properties and specificity of methionyl-tRNAfMet formyltransferase from Escherichia coli (1984), Methods Enzymol., 106, 141-152.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.2.9 EDTA or 2-mercaptoethanol stabilizes Escherichia coli
2.1.2.9 high ionic strength, e.g. 150 mM KCl stabilizes and prevents adsorption to glassware Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 0.00035
-
 L-methionyl-tRNAfMet
-
 Escherichia coli
2.1.2.9 0.0135
-
 10-formyltetrahydrofolate
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.9 cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.9 K+ activity is maximum in presence of 7 mM MgCl2 and 150 mM KCl Escherichia coli
2.1.2.9 Mg2+ activity is maximum in presence of 7 mM MgCl2 and 150 mM KCl Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.9 32000
-
 1 * 32000, SDS-PAGE Escherichia coli
2.1.2.9 33000
-
 sedimentation equilibrium method, gel filtration, small-angle neutron scattering Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O Escherichia coli involved in initiation of protein biosynthesis tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.1.2.9 Glycerol 50%, stable to Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Escherichia coli
-
-
-
2.1.2.9 Escherichia coli K12 EM20031(F32)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.9 affinity chromatography on Sepharose-bound tRNA Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.9 35.4
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.2.9 -15°C, 20 mM imidazole-HCl buffer, pH 7.6, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 50% glycerol, 150 mM KCl, several months, stable Escherichia coli
2.1.2.9 25°C, 20 mM imidazole-HCl buffer, pH 7.6, 0.1 mM EDTA, 10 mM 2-mercaptoethanol, 150 mM KCl, 2 days, stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O affinity of tRNAfMet for enzyme is improved by aminoacylation Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O 1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O involved in initiation of protein biosynthesis Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O affinity of tRNAfMet for enzyme is improved by aminoacylation Escherichia coli K12 EM20031(F32) tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O 1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme Escherichia coli K12 EM20031(F32) tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli K12 EM20031(F32) tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 additional information no formylation of methionyl-tRNAmMet Escherichia coli ?
-
 ?
2.1.2.9 additional information broad specificity at the level of tRNA binding, formylation of mischarged E. coli tRNAfMet Escherichia coli ?
-
 ?
2.1.2.9 additional information no formylation of methionyl-tRNAmMet Escherichia coli K12 EM20031(F32) ?
-
 ?
2.1.2.9 additional information broad specificity at the level of tRNA binding, formylation of mischarged E. coli tRNAfMet Escherichia coli K12 EM20031(F32) ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.9 monomer 1 * 32000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.9 25
-
 assay at Escherichia coli
2.1.2.9 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.9 20
-
 L-methionyl-tRNAfMet
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.9 10-formyltetrahydrofolate 1.1 mol 10-L-formyltetrahydrofolate is bound per mol monomeric enzyme Escherichia coli
2.1.2.9 tetrahydrofolate
-
 Escherichia coli