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Literature summary extracted from

  • Dickerman, H.W.; Steers, E.; Redfield, B.G.; Weissbach, H.
    Methionyl soluble ribonucleic acid transformylase. I. Purification and partial characterization (1967), J. Biol. Chem., 242, 1522-1525.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 0.01
-
 L-methionyl-tRNAMet
-
 Escherichia coli
2.1.2.9 0.0133
-
 10-formyltetrahydrofolate
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.9 cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.9 Ca2+ can partially replace Mg2+ Escherichia coli
2.1.2.9 K+ can partially replace Mg2+ Escherichia coli
2.1.2.9 Mg2+ requirement Escherichia coli
2.1.2.9 Mg2+ optimal concentration: 30 mM Escherichia coli
2.1.2.9 Na+ activates, can partially replace Mg2+ Escherichia coli
2.1.2.9 NH4+ activates, can partially replace Mg2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.9 25000
-
 high speed equilibrium sedimentation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Escherichia coli
-
-
-
2.1.2.9 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.9 over 1500fold purification Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.9 1.14
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.2.9 -20°C, 1 month, 30% loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet + H2O formylates methionyl-tRNAfMet from E. coli Escherichia coli B / ATCC 11303 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAMet
-
 Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAMet
-
 r
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAMet
-
 Escherichia coli B / ATCC 11303 tetrahydrofolate + N-formylmethionyl-tRNAMet
-
 r
2.1.2.9 additional information no formylation of deacylated heterogenous or deacylated partially purified methionyl-tRNA Escherichia coli ?
-
 ?
2.1.2.9 additional information no formyl group donor: 5,10-methenyltetrahydrofolate Escherichia coli ?
-
 ?
2.1.2.9 additional information no formylation of deacylated heterogenous or deacylated partially purified methionyl-tRNA Escherichia coli B / ATCC 11303 ?
-
 ?
2.1.2.9 additional information no formyl group donor: 5,10-methenyltetrahydrofolate Escherichia coli B / ATCC 11303 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.9 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.9 7.5
-
 with abrupt decrease of reaction rate below pH 7.0 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.9 6.8 8.4 about 60% of maximal activity at pH 6.8 and 8.4 Escherichia coli
2.1.2.9 7
-
 below: rapid decrease in reaction rate Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.9 10-formyltetrahydrofolate
-
 Escherichia coli
2.1.2.9 additional information no cofactors: ribonucleotide mono- or triphosphates Escherichia coli
2.1.2.9 additional information no cofactors: ATP, GTP Escherichia coli
2.1.2.9 tetrahydrofolate
-
 Escherichia coli