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Literature summary extracted from

  • Butler, M.M.; Graves, K.L.; Hardy, L.W.
    Evidence from 18O exchange studies for an exocyclic methylene intermediate in the reaction catalyzed by T4 deoxycytidylate hydroxymethylase (1994), Biochemistry, 33, 10521-10526.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.8 structural gene 42 encodes enzyme Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.8 C148G mutant is unable to catalyze a detectable 18O exchange in either the absence or presence of the folate cofactor Tequatrovirus T4
2.1.2.8 D179N mutant with altered substrate preference, favoring dUMP rather than dCMP Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.8 10-methyl-5,8-dideazatetrahydrofolate competitive inhibitor with respect to tetrahydrofolate Tequatrovirus T4
2.1.2.8 5-deazatetrahydrofolate competitive inhibitor with respect to tetrahydrofolate Tequatrovirus T4
2.1.2.8 5-fluorodeoxyuridylate
-
Tequatrovirus T4
2.1.2.8 5-hydroxymethyl-dCMP competitor of dCMP, inhibits formation of 5-hydroxymethyl-dCMP Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate Tequatrovirus T4
-
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.8 Tequatrovirus T4
-
Escherichia coli infected with bacteriophage T4
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate = tetrahydrofolate + 5-hydroxymethyldeoxycytidylate catalytic mechanism Tequatrovirus T4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.8 3.1
-
dCMP formation Tequatrovirus T4
2.1.2.8 4.6
-
5-hydroxymethyl-dCMP formation Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate 5-hydroxymethyl-dCMP is essential for DNA synthesis in phage T4 ?
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate Cys-148 is catalytically essential Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
r
2.1.2.8 additional information enzyme also catalyzes 18O exchange from solvent water into 5-hydroxymethyl-dCMP in the absence of exogenous tetrahydrofolate and in the presence of tetrahydrofolate analogues that lack N-5, mechanism Tequatrovirus T4 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.2.8 homodimer
-
Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.8 5,10-methylenetetrahydrofolate
-
Tequatrovirus T4
2.1.2.8 tetrahydrofolate
-
Tequatrovirus T4

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.2.8 0.01
-
10-methyl-5,8-dideazatetrahydrofolate
-
Tequatrovirus T4
2.1.2.8 0.1
-
5-hydroxymethyl-dCMP
-
Tequatrovirus T4
2.1.2.8 0.11
-
5-deazatetrahydrofolate
-
Tequatrovirus T4