Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • North, T.W.; Mathews, C.K.
    T4 phage-coded deoxycytidylate hydroxymethylase: purification and studies on intermolecular interactions (1977), Biochem. Biophys. Res. Commun., 77, 898-904.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.8 structural gene 42 encodes enzyme Tequatrovirus T4

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.8 additional information enzyme is no membrane component or very loosely attached to the membrane Tequatrovirus T4
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.8 27000
-
2 * 27000, SDS-PAGE Tequatrovirus T4
2.1.2.8 57000
-
gel filtration Tequatrovirus T4
2.1.2.8 63000
-
sedimentation equilibrium method Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate Tequatrovirus T4
-
tetrahydrofolate + 5-hydroxymethyldeoxycytidylate 5-hydroxymethyl-dCMP is a virus-specific DNA precursor ?
2.1.2.8 additional information Tequatrovirus T4 enzyme is part of a complex catalyzing DNA precursor biosynthesis ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.8 Tequatrovirus T4
-
Escherichia coli B infected with bacteriophage T4D and amber mutants
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.8 Escherichia coli B infected with Tequatrovirus T4

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.8 4.9
-
-
Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate
-
?
2.1.2.8 5,10-methylenetetrahydrofolate + H2O + deoxycytidylate
-
Tequatrovirus T4 tetrahydrofolate + 5-hydroxymethyldeoxycytidylate 5-hydroxymethyl-dCMP is a virus-specific DNA precursor ?
2.1.2.8 additional information enzyme is part of a complex catalyzing DNA precursor biosynthesis Tequatrovirus T4 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.2.8 dimer 2 * 27000, SDS-PAGE Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.8 5,10-methylenetetrahydrofolate
-
Tequatrovirus T4
2.1.2.8 tetrahydrofolate
-
Tequatrovirus T4