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Literature summary extracted from

  • Bashour, A.M.; Bloom, G.S.
    58K, a microtubule-binding Golgi protein, is a formiminotransferase cyclodeaminase (1998), J. Biol. Chem., 273, 19612-19617.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.5 cDNA encoding formiminotransferase cyclodeaminase is cloned and sequenced Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.5 Golgi membrane formiminotransferase cyclodeaminase, 58K, is associated with the cytoplasmic surface of the Golgi apparatus in vivo, cytoplasmically oriented peripheral membrane protein of the Golgi membranes Rattus norvegicus 139
-
2.1.2.5 microtubule formiminotransferase cyclodeaminase binds bovine brain microtubules, but not rat liver microtubules in vitro Rattus norvegicus 5874
-
4.3.1.4 Golgi apparatus Golgi apparatus, formiminotransferase-cyclodeaminase enzyme complex Rattus norvegicus 5794
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.5 480000
-
 approximately, native octameric bifunctional enzyme with formiminotransferase and cyclodeaminase activity, EC 2.1.2.5 and EC 4.3.1.4 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Rattus norvegicus bifunctional formiminotransferase cyclodeaminase, 58K, is associated with the cytoplasmatic surface of the Golgi apparatus in vivo 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate Rattus norvegicus histidine metabolism pathway, reaction occurs in vivo within the soluble phase of cytoplasm 5-formiminotetrahydrofolate + L-glutamate
-
 ?
4.3.1.4 5-formiminotetrahydrofolate Rattus norvegicus
-
 ?
-
 ?
4.3.1.4 additional information Rattus norvegicus folate-dependent bifunctional enzyme formiminoglutamate: tetrahydrofolate formiminotransferase-formiminotetrahydrofolate cyclodeaminase catalyzes two sequential reactions of the histidine degradation pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.5 Rattus norvegicus
-
-
-
4.3.1.4 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.5
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.5 liver richest tissue source of formiminotransferase cyclodeaminase, liver-specific enzyme Rattus norvegicus
-
2.1.2.5 additional information undetectable in adrenal gland, blood cells, marrow cells, brain, epididymis, heart, small intestine, kidney, lung, skeletal muscle, spleen and testis Rattus norvegicus
-
4.3.1.4 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.5 7
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.5 additional information 58K: formiminotransferase cyclodeaminase, bifunctional enzyme catalyzing two consecutive steps in the modification of tetrahydrofolate to 5,10-methenyl tetrahydrofolate Rattus norvegicus ?
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate bifunctional formiminotransferase cyclodeaminase, 58K, is associated with the cytoplasmatic surface of the Golgi apparatus in vivo Rattus norvegicus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 N-formiminoglutamate + tetrahydrofolate histidine metabolism pathway, reaction occurs in vivo within the soluble phase of cytoplasm Rattus norvegicus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate transfer of a single carbon from formiminoglutamate to tetrahydrofolate, product is 5-formiminotetrahydrofolate Rattus norvegicus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Rattus norvegicus 5,10-methenyltetrahydrofolate + NH3
-
 ?
4.3.1.4 5-formiminotetrahydrofolate
-
 Rattus norvegicus ?
-
 ?
4.3.1.4 additional information folate-dependent bifunctional enzyme formiminoglutamate: tetrahydrofolate formiminotransferase-formiminotetrahydrofolate cyclodeaminase catalyzes two sequential reactions of the histidine degradation pathway Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.5 homooctamer bifunctional formiminotransferase cyclodeaminase Rattus norvegicus
4.3.1.4 octamer 8* 58000, calculation from sequence of cDNA Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.5 30
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.5 tetrahydrofolate
-
 Rattus norvegicus