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Literature summary extracted from

  • Miller, A.; Waelsch, H.
    Formimino transfer from formamidinoglutaric acid to tetrahydrofolic acid (1957), J. Biol. Chem., 228, 397-417.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.2.5 no loss of activity after dialysis against phosphate buffer or 0.05 M EDTA, pH 7.4 Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.5 acetate inhibition in decreasing order: fumarate, succinate, citrate, acetate Bos taurus
2.1.2.5 acetyl-L-glutamate 0.015 mM, 30% inhibition Bos taurus
2.1.2.5 arsenate weak Bos taurus
2.1.2.5 Ca2+ 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ Bos taurus
2.1.2.5 citrate inhibition in decreasing order: fumarate, succinate, citrate, acetate Bos taurus
2.1.2.5 D-glutamate 0.015 mM, 47% inhibition Bos taurus
2.1.2.5 folic acid 0.3 mM, 20% inhibition Bos taurus
2.1.2.5 formyl-L-glutamate 0.015 mM, 53% inhibition Bos taurus
2.1.2.5 fumarate inhibition in decreasing order: fumarate, succinate, citrate, acetate Bos taurus
2.1.2.5 K+ 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ Bos taurus
2.1.2.5 K2SO4 0.1 M, 30% inhibition Bos taurus
2.1.2.5 KCl 0.1 M, 30% inhibition Bos taurus
2.1.2.5 KNO3 0.1 M, 65% inhibition Bos taurus
2.1.2.5 L-aspartate 0.015 mM, 26% inhibition Bos taurus
2.1.2.5 L-glutamate 0.015 mM, 93% inhibition Bos taurus
2.1.2.5 La3+ 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ Bos taurus
2.1.2.5 Li+ 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ Bos taurus
2.1.2.5 Mg2+ 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ Bos taurus
2.1.2.5 additional information not inhibited by formamidinoacetic acid, gamma-benzyl-formamidinoglutaric acid, formic acid, formamide, formylglutamine, formylisoglutamine Bos taurus
2.1.2.5 Na+ 0.1 M, strong inhibition in decreasing order: La3+, Ca2+, Mg2+, Li+, Na+, K+ Bos taurus
2.1.2.5 p-acetylaminobenzoylglutamate 0.3 mM, 50% inhibition Bos taurus
2.1.2.5 p-aminobenzoylglutamate 2.5 mM, 50% inhibition Bos taurus
2.1.2.5 p-formylaminobenzoylglutamate 0.3 mM, 50% inhibition Bos taurus
2.1.2.5 p-iodobenzoylglutamate 1.5 mM, 50% inhibition Bos taurus
2.1.2.5 phosphate weak Bos taurus
2.1.2.5 succinate inhibition in decreasing order: fumarate, succinate, citrate, acetate Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.5 soluble
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.5 NH4+ 0.1 M, 10% stimulation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.5 Bos taurus
-
 calf
-
2.1.2.5 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.5 60-80fold purification Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.5 5-formimidoyltetrahydrofolate + L-glutamate = tetrahydrofolate + N-formimidoyl-L-glutamate mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.5 kidney low activity Rattus norvegicus
-
2.1.2.5 kidney low activity Bos taurus
-
2.1.2.5 liver
-
 Rattus norvegicus
-
2.1.2.5 liver
-
 Bos taurus
-
2.1.2.5 additional information no activity in the soluble proteins of brain, heart, lung, testis, intestinal mucosa, diaphragm muscle or spleen Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.5 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.1.2.5 -15°C, crude extract, 1 year, stable Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.5 5-formyltetrahydrofolate + L-glutamate formation of N5-formyltetrahydrofolate + L-glutamate Bos taurus N-formyl-L-glutamate + tetrahydrofolate
-
 ?
2.1.2.5 additional information
-
 Rattus norvegicus ?
-
 ?
2.1.2.5 additional information primary reaction of the over-all reaction formimino-L-glutamate + tetrahydrofolate = N10-formyltetrahydrofolate + glutamate + NH3 Bos taurus ?
-
 ?
2.1.2.5 additional information not: formamidinoacetic acid, formylisoglutamine, formylglutamine, acetylglutamic acid Bos taurus ?
-
 ?
2.1.2.5 additional information formation of glutamic acid is reversible while formation of ammonia is not Bos taurus ?
-
 ?
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate
-
 Rattus norvegicus 5-formiminotetrahydrofolate + L-glutamate
-
 ?
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate alpha-L-formamidinoglutaric acid, absolute specificity with regard to the isomers of tetrahydrofolate at position 6, reduced pteroylaspartic acid and reduced pteroyltriglutamic acid can replace tetrahydrofolate Bos taurus 5-formiminotetrahydrofolate + L-glutamate
-
 r
2.1.2.5 tetrahydrofolate + N-formimino-L-glutamate specificity, equilibrium lies far on formation of formiminotetrahydrofolate and glutamate Bos taurus 5-formiminotetrahydrofolate + L-glutamate
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.5 6.5 9 unbuffered solution Bos taurus
2.1.2.5 6.5 7.6 phosphate buffer Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.5 6 9.8 about 50% of activity maximum at pH 6 and 9.8 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.5 tetrahydrofolate
-
 Rattus norvegicus
2.1.2.5 tetrahydrofolate
-
 Bos taurus