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Literature summary extracted from

  • Caperelli, C.A.
    Mammalian glycinamide ribonucleotide transformylase: Purification and some properties (1985), Biochemistry, 24, 1316-1320.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.2.2 medicine pharmacological importance, target enzyme for chemotherapy, development of novel antifolate drugs, to be used as anti-cancer drugs Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.2 0.00025
-
 10-formyl-5,8-dideazafolate
-
 Mus musculus
2.1.2.2 0.0008
-
 L-(-)-10-formyltetrahydrofolate
-
 Mus musculus
2.1.2.2 0.0019
-
 10-formyl-5,8-dideazafolate
-
 Gallus gallus
2.1.2.2 0.4
-
 glycinamide ribonucleotide
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.2 61000
-
 2 * 61000, SDS-PAGE Gallus gallus
2.1.2.2 102000
-
 1 * 102000, SDS-PAGE Mus musculus
2.1.2.2 110000
-
 1 * 110000, SDS-PAGE Mus musculus
2.1.2.2 125000
-
 sucrose density gradient ultracentrifugation Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Mus musculus in ureotelic animals such as mammals the role of the far less active pathway is to provide purine nucleotides for further anabolic sequences tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Gallus gallus in uricotelic organisms such as birds, the major role of this active pathway is elimination of waste nitrogen tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Gallus gallus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide Mus musculus essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.2 Gallus gallus
-
 chicken
-
2.1.2.2 Mus musculus
-
 mouse
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.2
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.2 L-5178-Y cell murine lymphoma cell line Mus musculus
-
2.1.2.2 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.2 3.3
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.2 10-formyl-5,8-dideazafolate + carbocyclic beta-glycinamide ribonucleotide
-
 Mus musculus 5,8-dideazafolate + N-formyl carbocyclic beta-glycinamide ribonucleotide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide
-
 Mus musculus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide in ureotelic animals such as mammals the role of the far less active pathway is to provide purine nucleotides for further anabolic sequences Mus musculus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide in uricotelic organisms such as birds, the major role of this active pathway is elimination of waste nitrogen Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Gallus gallus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 10-formyltetrahydrofolate + 5-phospho-D-ribosylglycinamide essential enzyme in DNA synthesis, third enzyme in de novo purine biosynthesis pathway Mus musculus tetrahydrofolate + 5'-phosphoribosyl-N-formylglycinamide
-
 ?
2.1.2.2 5'-phosphoribosylglycinamide + 10-formyl-5,8-dideazafolate
-
 Gallus gallus 5'-phosphoribosyl-N-formylglycinamide + 5,8-dideazafolate
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.2 dimer 2 * 61000, SDS-PAGE Gallus gallus
2.1.2.2 monomer
-
 Gallus gallus
2.1.2.2 monomer 1 * 110000, SDS-PAGE Mus musculus
2.1.2.2 monomer 1 * 102000, SDS-PAGE Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.2 10-formyl-5,8-dideazafolate
-
 Mus musculus