EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.2.11 | panB gene encoding enzyme is cloned, sequenced and 50fold overexpressed in Escherichia coli panB mutant Hfr3000 YA139 containing plasmid pCEJ01, gene is 792 bp long and encodes a protein of 264 amino acids, gene is likely to be cotranscribed with at least one other gene, panB, panC and panD genes are closely clustered at 3.1 min of the Escherichia coli K12 genetic map | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.2.11 | additional information | panB mutant Hfr3000 YA139 completely lacks KPHMT | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.11 | 0.15 | - |
ketopantoate | - |
Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | 28179 | - |
6 * 28179, calculated from the amino acid sequence and electrospray mass spectrometry, 6 * 29000, recombinant enzyme expressed in Escherichia coli Hfr3000 YA139/pCEJ01, SDS-PAGE | Escherichia coli |
2.1.2.11 | 29000 | - |
6 * 28179, calculated from the amino acid sequence and electrospray mass spectrometry, 6 * 29000, recombinant enzyme expressed in Escherichia coli Hfr3000 YA139/pCEJ01, SDS-PAGE | Escherichia coli |
2.1.2.11 | 174000 | - |
recombinant enzyme, gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Escherichia coli | first committed step in pantothenate biosynthesis | tetrahydrofolate + 2-dehydropantoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.11 | Escherichia coli | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.1.2.11 | no modification | - |
Escherichia coli |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.11 | 19.3fold purification of recombinant enzyme 50fold overexpressed in E. coli panB mutant Hfr3000 YA139/pCEJ01 | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | 0.007 | - |
strain K12 | Escherichia coli |
2.1.2.11 | 0.162 | - |
strain Hfr3000 YA139/pCEJ02 | Escherichia coli |
2.1.2.11 | 0.348 | - |
strain Hfr3000 YA139/pCEJ01 | Escherichia coli |
2.1.2.11 | 0.666 | - |
strain Hfr3000 YA139/pSAL38 | Escherichia coli |
2.1.2.11 | 3890 | - |
purified recombinant enzyme from strain Hfr3000 YA139/pCEJ01 | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | - |
Escherichia coli | tetrahydrofolate + 2-dehydropantoate | - |
r | |
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | first committed step in pantothenate biosynthesis | Escherichia coli | tetrahydrofolate + 2-dehydropantoate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.2.11 | hexamer | 6 * 28179, calculated from the amino acid sequence and electrospray mass spectrometry, 6 * 29000, recombinant enzyme expressed in Escherichia coli Hfr3000 YA139/pCEJ01, SDS-PAGE | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.11 | 5,10-methylenetetrahydrofolate | - |
Escherichia coli | |
2.1.2.11 | tetrahydrofolate | - |
Escherichia coli |