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Literature summary extracted from

  • Kim, J.K.; Kim, K.D.; Lim, J.S.; Lee, H.G.; Kim, S.J.; Cho, S.H.; Jeong, W.H.; Choe, I.S.; Chung, T.W.; Paik, S.G.; Choe, Y.K.
    Cloning and characterization of the Mycobacterium bovis BCG panB gene encoding ketopantoate hydroxymethyltransferase (2001), J. Biochem. Mol. Biol., 34, 342-346.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.11 panB gene encoding enzyme is cloned, sequenced and overexpressed in Escherichia coli BL21, ORF of 846 bp encodes a protein of 281 amino acids, N-terminal 37 amino acids are essential for enzyme function Mycobacterium tuberculosis variant bovis
2.1.2.11 PCR amplification of panB gene, which is identical to that of Mycobacterium bovis BCG Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.11 soluble
-
Mycobacterium tuberculosis
-
-
2.1.2.11 soluble most of recombinant enzyme is found in a soluble fraction Mycobacterium tuberculosis variant bovis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.11 29337
-
x * 29337, calculated from the amino acid sequence, x * 35000, recombinant enzyme expressed in Escherichia coli BL21, SDS-PAGE Mycobacterium tuberculosis variant bovis
2.1.2.11 35000
-
x * 29337, calculated from the amino acid sequence, x * 35000, recombinant enzyme expressed in Escherichia coli BL21, SDS-PAGE Mycobacterium tuberculosis variant bovis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate Mycobacterium tuberculosis first committed step in pantothenate biosynthesis tetrahydrofolate + 2-dehydropantoate
-
?
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate Mycobacterium tuberculosis variant bovis first committed step in pantothenate biosynthesis tetrahydrofolate + 2-dehydropantoate
-
?
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate Mycobacterium tuberculosis KIT 10468 first committed step in pantothenate biosynthesis tetrahydrofolate + 2-dehydropantoate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.11 Mycobacterium tuberculosis
-
-
-
2.1.2.11 Mycobacterium tuberculosis KIT 10468
-
-
-
2.1.2.11 Mycobacterium tuberculosis variant bovis
-
BCG, strain 1173-P2, live vaccine against tuberculosis, used for superficial bladder cancer immunotherapy
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.11 purification of recombinant enzyme overexpressed in Escherichia coli BL21 Mycobacterium tuberculosis variant bovis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.11 0.019
-
recombinant enzyme Mycobacterium tuberculosis variant bovis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate
-
Mycobacterium tuberculosis tetrahydrofolate + 2-dehydropantoate
-
?
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate
-
Mycobacterium tuberculosis variant bovis tetrahydrofolate + 2-dehydropantoate
-
?
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate first committed step in pantothenate biosynthesis Mycobacterium tuberculosis tetrahydrofolate + 2-dehydropantoate
-
?
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate first committed step in pantothenate biosynthesis Mycobacterium tuberculosis variant bovis tetrahydrofolate + 2-dehydropantoate
-
?
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate
-
Mycobacterium tuberculosis KIT 10468 tetrahydrofolate + 2-dehydropantoate
-
?
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate first committed step in pantothenate biosynthesis Mycobacterium tuberculosis KIT 10468 tetrahydrofolate + 2-dehydropantoate
-
?

Subunits

EC Number Subunits Comment Organism
2.1.2.11 ? x * 29337, calculated from the amino acid sequence, x * 35000, recombinant enzyme expressed in Escherichia coli BL21, SDS-PAGE Mycobacterium tuberculosis variant bovis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.11 37
-
assay at Mycobacterium tuberculosis
2.1.2.11 37
-
assay at Mycobacterium tuberculosis variant bovis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.11 5,10-methylenetetrahydrofolate
-
Mycobacterium tuberculosis
2.1.2.11 5,10-methylenetetrahydrofolate
-
Mycobacterium tuberculosis variant bovis
2.1.2.11 tetrahydrofolate
-
Mycobacterium tuberculosis
2.1.2.11 tetrahydrofolate
-
Mycobacterium tuberculosis variant bovis