EC Number | General Stability | Organism |
---|---|---|
2.1.2.11 | not inactivated by borohydride reduction in the presence of excess substrates | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.11 | coenzyme A | above 1 mM | Escherichia coli | |
2.1.2.11 | formaldehyde | 0.8 mM, partial inhibition; forward reaction | Escherichia coli | |
2.1.2.11 | Pantoate | 0.05 mM or above | Escherichia coli | |
2.1.2.11 | pantothenate | 0.5 mM or above | Escherichia coli | |
2.1.2.11 | tetrahydrofolate | 0.38 mM, partial inhibition; forward reaction | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.11 | 0.18 | - |
tetrahydrofolate | - |
Escherichia coli | |
2.1.2.11 | 1.1 | - |
3-methyl-2-oxobutanoate | - |
Escherichia coli | |
2.1.2.11 | 5.9 | - |
formaldehyde | - |
Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.11 | Co2+ | requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active | Escherichia coli | |
2.1.2.11 | Mg2+ | requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active | Escherichia coli | |
2.1.2.11 | Mn2+ | requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active | Escherichia coli | |
2.1.2.11 | Zn2+ | requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | 25700 | - |
10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis | Escherichia coli |
2.1.2.11 | 27000 | - |
10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis | Escherichia coli |
2.1.2.11 | 255000 | - |
sedimentation equilibrium | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Escherichia coli | first committed step in pantothenate biosynthesis | tetrahydrofolate + 2-dehydropantoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.11 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.11 | strain K12, 2450fold purification | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate | class II aldolase | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | 7.3 | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.2.11 | 4°C, 100 mM potassium phosphate, pH 6.8, 1 mM EDTA, 0.5 mM dithiothreitol, 6 months, almost no loss of activity | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.11 | 5,10-methylenetetrahydrofolate + 2-oxobutyrate | - |
Escherichia coli | ? | - |
? | |
2.1.2.11 | 5,10-methylenetetrahydrofolate + 2-oxopentanoate | - |
Escherichia coli | ? | - |
? | |
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | - |
Escherichia coli | tetrahydrofolate + 2-dehydropantoate | synthesis of ketopantoate, the following components can replace tetrahydrofolate: tetrahydropteroylmono-, di-, tri-, tetra-, penta-, hexa-, and heptaglutamate, absolute requirement for tetrahydrofolate, only the L-isomer is active | r | |
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | first committed step in pantothenate biosynthesis | Escherichia coli | tetrahydrofolate + 2-dehydropantoate | - |
? | |
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxopentanoate | - |
Escherichia coli | ? | - |
? | |
2.1.2.11 | formaldehyde + tetrahydrofolate | - |
Escherichia coli | methylentetrahydrofolate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.2.11 | decamer | 10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | 37 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | additional information | - |
pI: 4.4 | Escherichia coli |
2.1.2.11 | 7 | 7.6 | - |
Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | additional information | - |
stable and active over a broad pH-range | Escherichia coli |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | additional information | - |
stable and active over a broad pH-range | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.11 | 5,10-methylenetetrahydrofolate | - |
Escherichia coli | |
2.1.2.11 | tetrahydrofolate | requires tetrahydrofolate | Escherichia coli |