Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Powers, S.G.; Snell, E.E.
    Purification and properties of ketopantoate hydroxymethyltransferase (1979), Methods Enzymol., 62, 204-209.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.2.11 not inactivated by borohydride reduction in the presence of excess substrates Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.2.11 coenzyme A above 1 mM Escherichia coli
2.1.2.11 formaldehyde 0.8 mM, partial inhibition; forward reaction Escherichia coli
2.1.2.11 Pantoate 0.05 mM or above Escherichia coli
2.1.2.11 pantothenate 0.5 mM or above Escherichia coli
2.1.2.11 tetrahydrofolate 0.38 mM, partial inhibition; forward reaction Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.11 0.18
-
tetrahydrofolate
-
Escherichia coli
2.1.2.11 1.1
-
3-methyl-2-oxobutanoate
-
Escherichia coli
2.1.2.11 5.9
-
formaldehyde
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.11 Co2+ requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active Escherichia coli
2.1.2.11 Mg2+ requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active Escherichia coli
2.1.2.11 Mn2+ requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active Escherichia coli
2.1.2.11 Zn2+ requires Mg2+, Mn2+, Co2+ and Zn2+ are progressively less active Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.11 25700
-
10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis Escherichia coli
2.1.2.11 27000
-
10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis Escherichia coli
2.1.2.11 255000
-
sedimentation equilibrium Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate Escherichia coli first committed step in pantothenate biosynthesis tetrahydrofolate + 2-dehydropantoate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.11 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.11 strain K12, 2450fold purification Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate class II aldolase Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.11 7.3
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.2.11 4°C, 100 mM potassium phosphate, pH 6.8, 1 mM EDTA, 0.5 mM dithiothreitol, 6 months, almost no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.11 5,10-methylenetetrahydrofolate + 2-oxobutyrate
-
Escherichia coli ?
-
?
2.1.2.11 5,10-methylenetetrahydrofolate + 2-oxopentanoate
-
Escherichia coli ?
-
?
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate
-
Escherichia coli tetrahydrofolate + 2-dehydropantoate synthesis of ketopantoate, the following components can replace tetrahydrofolate: tetrahydropteroylmono-, di-, tri-, tetra-, penta-, hexa-, and heptaglutamate, absolute requirement for tetrahydrofolate, only the L-isomer is active r
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate first committed step in pantothenate biosynthesis Escherichia coli tetrahydrofolate + 2-dehydropantoate
-
?
2.1.2.11 5,10-methylenetetrahydrofolate + 3-methyl-2-oxopentanoate
-
Escherichia coli ?
-
?
2.1.2.11 formaldehyde + tetrahydrofolate
-
Escherichia coli methylentetrahydrofolate
-
?

Subunits

EC Number Subunits Comment Organism
2.1.2.11 decamer 10 * 27000, SDS-PAGE, 10 * 25700, amino acid analysis Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.11 37
-
assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.11 additional information
-
pI: 4.4 Escherichia coli
2.1.2.11 7 7.6
-
Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.2.11 additional information
-
stable and active over a broad pH-range Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.2.11 additional information
-
stable and active over a broad pH-range Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.11 5,10-methylenetetrahydrofolate
-
Escherichia coli
2.1.2.11 tetrahydrofolate requires tetrahydrofolate Escherichia coli