EC Number | General Stability | Organism |
---|---|---|
2.1.2.11 | ammonium sulfate fractionation inactivates, resistant to urea denaturation | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.2.11 | additional information | - |
additional information | low Km-values for its substrates | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Salmonella enterica subsp. enterica serovar Typhimurium | first committed step in pantothenate biosynthesis | tetrahydrofolate + 2-dehydropantoate | - |
? | |
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | Escherichia coli | first committed step in pantothenate biosynthesis | tetrahydrofolate + 2-dehydropantoate | enzyme is responsible for catalysis of ketopantoate formation in vivo | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.2.11 | Escherichia coli | - |
- |
- |
2.1.2.11 | Escherichia coli | - |
strains W and B | - |
2.1.2.11 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.2.11 | strain K12, 2450fold purification | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | 7.3 | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | tetrahydrofolate + 2-dehydropantoate | - |
r | |
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | - |
Escherichia coli | tetrahydrofolate + 2-dehydropantoate | formation of ketopantoate, syn. 2-keto-3,3-dimethyl-4-hydroxybutyrate, tetrahydrofolate-dependent enzyme | r | |
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | first committed step in pantothenate biosynthesis | Salmonella enterica subsp. enterica serovar Typhimurium | tetrahydrofolate + 2-dehydropantoate | - |
? | |
2.1.2.11 | 5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate | first committed step in pantothenate biosynthesis | Escherichia coli | tetrahydrofolate + 2-dehydropantoate | enzyme is responsible for catalysis of ketopantoate formation in vivo | ? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | 37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
2.1.2.11 | 37 | - |
assay at | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | 55 | - |
treatment at 55°C largely destroys enzyme in crude extract, partially purified enzyme is more heat-stabile | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | 8 | - |
at or below | Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.2.11 | 10 | - |
no ketopantoate formation above pH 10 | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.2.11 | 5,10-methylenetetrahydrofolate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2.1.2.11 | 5,10-methylenetetrahydrofolate | - |
Escherichia coli | |
2.1.2.11 | tetrahydrofolate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2.1.2.11 | tetrahydrofolate | tetrahydrofolate-dependent reaction | Escherichia coli |