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Literature summary extracted from

  • Fawaz, F.; Jones, G.H.
    Actinomycin synthesis in Streptomyces antibioticus (1988), J. Biol. Chem., 263, 4602-4606.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.97 2-mercaptoethanol
-
 Streptomyces antibioticus
2.1.1.97 Na2EDTA
-
 Streptomyces antibioticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.97 0.18
-
 S-adenosyl-L-methionine
-
 Streptomyces antibioticus
2.1.1.97 0.64
-
 3-Hydroxyanthranilate
-
 Streptomyces antibioticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.97 36000
-
 1 * 36000, SDS-PAGE Streptomyces antibioticus
2.1.1.97 37000
-
 gel filtration, chromatography in 0.5 M NaCl Streptomyces antibioticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.97 S-adenosyl-L-methionine + 3-hydroxyanthranilate Streptomyces antibioticus involved in the biosynthesis of the antibiotic actinomycin S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.97 Streptomyces antibioticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.97
-
 Streptomyces antibioticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.97 mycelium
-
 Streptomyces antibioticus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.97 3.052
-
-
 Streptomyces antibioticus

Storage Stability

EC Number Storage Stability Organism
2.1.1.97 -70°C, 5% glycerol, 50 mM sodium phosphate, pH 7.0, 10 mM Na2EDTA, 5 mM 2-mercaptoethanol, 1 mM phenylmethylsulfonyl fluoride, quite stable, can be frozen and thawed several times without appreciable loss of activity Streptomyces antibioticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.97 S-adenosyl-L-methionine + 3-hydroxyanthranilate involved in the biosynthesis of the antibiotic actinomycin Streptomyces antibioticus S-adenosyl-L-homocysteine + 3-hydroxy-4-methylanthranilate
-
 ?
2.1.1.97 S-adenosyl-L-methionine + 3-hydroxyanthranilic acid
-
 Streptomyces antibioticus S-adenosyl-L-homocysteine + 4-methyl-3-hydroxyanthranilic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.97 monomer 1 * 36000, SDS-PAGE Streptomyces antibioticus