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Literature summary extracted from

  • Sauer, K.; Thauer, R.K.
    Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Zinc dependence and thermodynamics of the methanol:cob(I)alamin methyltransferase reaction (1997), Eur. J. Biochem., 249, 280-285.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.90 expression of beta subunit MtaB in Escherichia coli Methanosarcina barkeri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.90 40
-
 methanol beta subunit MtaB Methanosarcina barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.90 Zn2+ beta subunit MtaB contains 1 mol Zn2+/mol subunit, Zn2+ can be substituted by Co2+ Methanosarcina barkeri
2.1.1.246 Zn2+ dependent on, kinetics, overview Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.246 additional information Methanosarcina barkeri transferase MtaA catalyzes the transfer of the methyl group from methylated transferase MtaBC to coenzyme M. Instead of methylated transferase MtaBC, MtaA can also use free methylcobalamin as the methyl donor. MtaA activates coenzyme M for nucleophilic attack of the methyl group of the methylcobamide ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.90 Methanosarcina barkeri Q46EH3
-
-
2.1.1.246 Methanosarcina barkeri
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.90 methanol + cob(I)alamin catalyzed by beta subunit MtaB alone Methanosarcina barkeri methyl-cob(III)alamin + H2O
-
 r
2.1.1.246 additional information transferase MtaA catalyzes the transfer of the methyl group from methylated transferase MtaBC to coenzyme M. Instead of methylated transferase MtaBC, MtaA can also use free methylcobalamin as the methyl donor. MtaA activates coenzyme M for nucleophilic attack of the methyl group of the methylcobamide Methanosarcina barkeri ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.246 methanol:cob(I)alamin methyltransferase
-
 Methanosarcina barkeri
2.1.1.246 mtaA
-
 Methanosarcina barkeri

General Information

EC Number General Information Comment Organism
2.1.1.246 metabolism the enzyme system from Methanosarcina barkeri is composed of two methyltransferases, transferase MT1, which is composed of a 50-kDa subunit, MtaB, and a 27-kDa corrinoid-harbouring subunit, MtaC, catalyzes the methylation of free cob(1)alamin with methanol, EC 2.1.1.90, and transferase MT2 catalyzes the transfer of the methyl group from CH,-MT1 to coenzyme M. Instead of CH,-MTI, MtaA can also use free methylcobalamin as the methyl donor. MtaC with its supernucleophilic cobamide prosthetic group accepts the methyl group from methanol and passes it to coenzyme M, MtaB activates methanol for nucleophilic attack by the cob(1)amide, and MtaA activates coenzyme M for nucleophilic attack of the methyl group of the methylcobamide Methanosarcina barkeri