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Literature summary extracted from

  • Van Brederode, J.; Kamps-Heinsbroek, R.; Mastenbroek, O.
    Biochemical and ontogenetic evidence that the ferulic acid and isocoparin formation in silene are catalyzed by different enzymes (1982), Z. Pflanzenphysiol., 106, 43-53.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.78 EDTA
-
 Silene latifolia subsp. alba
2.1.1.78 Hg2+
-
 Silene latifolia subsp. alba
2.1.1.78 Zn2+
-
 Silene latifolia subsp. alba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.78 0.001
-
 caffeic acid second substrate caffeic acid Silene latifolia subsp. alba
2.1.1.78 0.0012
-
 S-adenosyl-L-methionine second substrate caffeic acid Silene latifolia subsp. alba
2.1.1.78 0.004
-
 S-adenosyl-L-methionine second substrate isoorientin Silene latifolia subsp. alba
2.1.1.78 0.005
-
 S-adenosyl-L-methionine second substrate isoorientin 2''-O-rhamnoside Silene latifolia subsp. alba
2.1.1.78 0.007
-
 isoorientin 2''-O-rhamnoside second substrate S-adenosyl-L-methionine Silene latifolia subsp. alba
2.1.1.78 0.32
-
 isoorientin
-
 Silene latifolia subsp. alba

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.78 Co2+ slightly stimulatory Silene latifolia subsp. alba
2.1.1.78 Mn2+ slightly stimulatory Silene latifolia subsp. alba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.78 S-adenosyl-L-methionine + isoorientin Silene latifolia subsp. alba involved in biosynthesis of flavones S-adenosyl-L-homocysteine + isoscoparin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.78 Silene latifolia subsp. alba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.78 partial Silene latifolia subsp. alba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.78 leaf
-
 Silene latifolia subsp. alba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.78 S-adenosyl-L-methionine + caffeic acid
-
 Silene latifolia subsp. alba ?
-
 ?
2.1.1.78 S-adenosyl-L-methionine + isoorientin
-
 Silene latifolia subsp. alba S-adenosyl-L-homocysteine + isoscoparin
-
 ?
2.1.1.78 S-adenosyl-L-methionine + isoorientin involved in biosynthesis of flavones Silene latifolia subsp. alba S-adenosyl-L-homocysteine + isoscoparin
-
 ?
2.1.1.78 S-adenosyl-L-methionine + isoorientin 2''-O-rhamnoside
-
 Silene latifolia subsp. alba S-adenosyl-L-homocysteine + isoscoparin 2''-O-rhamnoside
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.78 45
-
-
 Silene latifolia subsp. alba

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.78 30 55 35% of maximum activity at 30°C and 55°C Silene latifolia subsp. alba

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.78 40
-
 after 20 min: 55% activity remains, after 40 min 20% activity remains Silene latifolia subsp. alba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.78 8
-
 isoorientin as substrate Silene latifolia subsp. alba

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.78 7 9
-
 Silene latifolia subsp. alba