EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.76 | 2-mercaptoethanol | relative activity 105% | Chrysosplenium americanum | |
2.1.1.76 | iodoacetamide | relative activity 105% | Chrysosplenium americanum |
EC Number | General Stability | Organism |
---|---|---|
2.1.1.82 | loss of activity during chromatofocusing | Chrysosplenium americanum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.76 | N-ethylmaleimide | - |
Chrysosplenium americanum | |
2.1.1.76 | p-chloromercuribenzoate | - |
Chrysosplenium americanum | |
2.1.1.82 | N-ethylmaleimide | - |
Chrysosplenium americanum | |
2.1.1.82 | p-chloromercuribenzoate | - |
Chrysosplenium americanum | |
2.1.1.83 | N-ethylmaleimide | - |
Chrysosplenium americanum | |
2.1.1.83 | p-chloromercuribenzoate | - |
Chrysosplenium americanum | |
2.1.1.84 | EDTA | - |
Chrysosplenium americanum | |
2.1.1.84 | N-ethylmaleimide | - |
Chrysosplenium americanum | |
2.1.1.84 | p-chloromercuribenzoate | - |
Chrysosplenium americanum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.76 | K+ | relative activity 115% | Chrysosplenium americanum | |
2.1.1.76 | Mn2+ | relative activity 110% | Chrysosplenium americanum | |
2.1.1.84 | Mg2+ | activates | Chrysosplenium americanum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.76 | 57000 | - |
gel filtration | Chrysosplenium americanum |
2.1.1.76 | 65000 | - |
gel filtration | Chrysosplenium americanum |
2.1.1.82 | 57000 | - |
gel filtration | Chrysosplenium americanum |
2.1.1.83 | 57000 | - |
gel filtration | Chrysosplenium americanum |
2.1.1.84 | 57000 | - |
gel filtration | Chrysosplenium americanum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.76 | S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone | Citrofortunella microcarpa | - |
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone | Chrysosplenium americanum | - |
S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone | 3-O-methylquercetin | ? | |
2.1.1.82 | S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxy-3-methoxyflavone | Chrysosplenium americanum | involved with EC 2.1.1.76 and 2.1.1.83 in the methylation of quercetin | S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,7-dimethoxyflavone | trivial name 3,7-dimethylquercetin | ? | |
2.1.1.83 | S-adenosyl-L-methionine + 3',4',5-trihydroxy-3,7-dimethoxyflavone | Chrysosplenium americanum | trivial name 3,7-dimethylquercetin, enzyme does not act on flavones, dihydroflavonols or their glucosides, involved with EC 2.1.1.76 and 2.1.1.82 in the methylation of quercitin to 3,7,4'-trimethylquercitin | S-adenosyl-L-homocysteine + 3',5-dihydroxy-3,4',7-trimethoxyflavone | trivial name 3,7,4'-trimethylquercetin | ? | |
2.1.1.84 | S-adenosyl-L-methionine + 3',4',5,6-tetrahydroxy-3,7-dimethoxyflavone | Chrysosplenium americanum | - |
S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,6,7-trimethoxyflavone | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.76 | Chrysosplenium americanum | - |
- |
- |
2.1.1.76 | Citrofortunella microcarpa | - |
calamondin orange, synonym Citrofortunella mitis | - |
2.1.1.82 | Chrysosplenium americanum | - |
- |
- |
2.1.1.83 | Chrysosplenium americanum | - |
- |
- |
2.1.1.84 | Chrysosplenium americanum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.76 | partially | Chrysosplenium americanum |
2.1.1.76 | partially | Citrofortunella microcarpa |
2.1.1.82 | loss of activity during chromatofocusing possibly due to instability of the enzyme in the purified form or to the loss of some factors required for activity | Chrysosplenium americanum |
2.1.1.83 | - |
Chrysosplenium americanum |
2.1.1.84 | ammonium sulfate, Sephacryl S-200, hydroxylapatite, chromatofocusing | Chrysosplenium americanum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.82 | leaf | - |
Chrysosplenium americanum | - |
2.1.1.83 | leaf | - |
Chrysosplenium americanum | - |
2.1.1.84 | leaf | - |
Chrysosplenium americanum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.76 | additional information | - |
53 pkat/mg | Chrysosplenium americanum |
2.1.1.83 | 7.2 | - |
- |
Chrysosplenium americanum |
2.1.1.84 | 2.64 | - |
- |
Chrysosplenium americanum |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.1.76 | -15°C, purified enzyme, 0.2 M potassium phosphate, pH 8.0, 20% glycerol, 5 mM dithiothreitol, stable for several months | Chrysosplenium americanum |
2.1.1.76 | 4°C, partially purified enzyme loses 80% of its activity over a period of 4-6 weeks | Chrysosplenium americanum |
2.1.1.82 | -15°C, 200 mM potassium phosphate buffer, pH 8, 20% v/v glycerol, 5 mM dithiothreitol, several months, no loss of activity | Chrysosplenium americanum |
2.1.1.82 | 4°C, 4-6 weeks, 80% loss of activity | Chrysosplenium americanum |
2.1.1.83 | -15°C, 200 mM potassium phosphate buffer, pH 8, 20% glycerol, 5 mM dithiothreitol, several months, no loss of activity | Chrysosplenium americanum |
2.1.1.83 | 4°C, 4-6 weeks, 80% loss of activity | Chrysosplenium americanum |
2.1.1.84 | -15°C, 0.2 M potassium phosphate, pH 8, 20% glycerol, 5 mM dithiothreitol, for several months, no loss of activity | Chrysosplenium americanum |
2.1.1.84 | 4°C, 4-6 weeks, 80% loss of activity | Chrysosplenium americanum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.76 | S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone | - |
Chrysosplenium americanum | S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone | - |
Citrofortunella microcarpa | S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone | - |
? | |
2.1.1.76 | S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone | - |
Chrysosplenium americanum | S-adenosyl-L-homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone | 3-O-methylquercetin | ? | |
2.1.1.76 | S-adenosyl-L-methionine + 7-methylquercetin | i.e. rhamnetin, 30% of the activity with quercetin | Chrysosplenium americanum | ? | - |
? | |
2.1.1.82 | S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxy-3-methoxyflavone | trivial name 3-methylquercetin, specific for 3-methylquercetin | Chrysosplenium americanum | S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,7-dimethoxyflavone | trivial name 3,7-dimethylquercetin | ? | |
2.1.1.82 | S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxy-3-methoxyflavone | involved with EC 2.1.1.76 and 2.1.1.83 in the methylation of quercetin | Chrysosplenium americanum | S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,7-dimethoxyflavone | trivial name 3,7-dimethylquercetin | ? | |
2.1.1.83 | S-adenosyl-L-methionine + 3',4',5-trihydroxy-3,7-dimethoxyflavone | trivial name 3,7-dimethylquercetin, enzyme does not act on flavones, dihydroflavonols or their glucosides | Chrysosplenium americanum | S-adenosyl-L-homocysteine + 3',5-dihydroxy-3,4',7-trimethoxyflavone | trivial name 3,7,4'-trimethylquercetin | ? | |
2.1.1.83 | S-adenosyl-L-methionine + 3',4',5-trihydroxy-3,7-dimethoxyflavone | trivial name 3,7-dimethylquercetin, enzyme does not act on flavones, dihydroflavonols or their glucosides, involved with EC 2.1.1.76 and 2.1.1.82 in the methylation of quercitin to 3,7,4'-trimethylquercitin | Chrysosplenium americanum | S-adenosyl-L-homocysteine + 3',5-dihydroxy-3,4',7-trimethoxyflavone | trivial name 3,7,4'-trimethylquercetin | ? | |
2.1.1.83 | S-adenosyl-L-methionine + 3,7-dimethylquercetagetin | - |
Chrysosplenium americanum | S-adenosyl-L-homocysteine + 3,7,4'-trimethylquercetagetin + H+ | - |
? | |
2.1.1.84 | 3,7,3'-trimethylquercetagetin + S-adenosyl-L-methionine | - |
Chrysosplenium americanum | 3,6,7',3'-tetrametylquercetagetin + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.84 | 8-hydroxykaempferol + S-adenosyl-L-methionine | 25% of activity with 3',4',5,6-tetrahydroxy-3,7-dimethoxyflavone | Chrysosplenium americanum | ? | - |
? | |
2.1.1.84 | S-adenosyl-L-methionine + 3',4',5,6-tetrahydroxy-3,7-dimethoxyflavone | - |
Chrysosplenium americanum | S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-3,6,7-trimethoxyflavone | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.76 | 7.8 | 8.2 | - |
Chrysosplenium americanum |
2.1.1.82 | 7.8 | 8.2 | - |
Chrysosplenium americanum |
2.1.1.83 | 8.8 | 9 | - |
Chrysosplenium americanum |
2.1.1.84 | 8.8 | 9 | - |
Chrysosplenium americanum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.76 | 7.2 | 9.3 | about 50% of activity maximum at pH 7.2 and 9.3 | Chrysosplenium americanum |
2.1.1.82 | 7.2 | 9.5 | approx. 50% of maximal activity at pH 7.2 and 9.5 | Chrysosplenium americanum |
2.1.1.83 | 7.2 | 9.5 | approx. 50% of maximal activity at pH 7.2 and 9.5 | Chrysosplenium americanum |