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Literature summary extracted from

  • Kuroki, G.; Poulton, J.E.
    The para-O-methylation of apigenin to acacetin by cell-free extracts of Robinia pseudoacacia L. (1981), Z. Naturforsch. C, 36c, 916-920.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.75 2-mercaptoethanol 5 mM, required for optimum activity Robinia pseudoacacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.75 S-adenosyl-L-homocysteine 0.1 mM, 47% inhibition Robinia pseudoacacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.75 0.031
-
 S-adenosyl-L-methionine
-
 Robinia pseudoacacia
2.1.1.75 0.05
-
 apigenin
-
 Robinia pseudoacacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.75 S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone Robinia pseudoacacia enzyme is involved in biosynthesis of 4'-methoxylated flavonoids S-adenosyl-L-homocysteine + 4'-methoxy-5,7-dihydroxyflavone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.75 Robinia pseudoacacia
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.75 callus
-
 Robinia pseudoacacia
-
2.1.1.75 seedling
-
 Robinia pseudoacacia
-
2.1.1.75 shoot
-
 Robinia pseudoacacia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.75 S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone i.e. apigenin Robinia pseudoacacia S-adenosyl-L-homocysteine + 4'-methoxy-5,7-dihydroxyflavone i.e. acacetin ?
2.1.1.75 S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone enzyme is involved in biosynthesis of 4'-methoxylated flavonoids Robinia pseudoacacia S-adenosyl-L-homocysteine + 4'-methoxy-5,7-dihydroxyflavone
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.75 9
-
 glycine-NaOH buffer Robinia pseudoacacia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.75 7.5 10 about 50% of activity maximum at pH 7.5 and 10, glycine-NaOH buffer Robinia pseudoacacia