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Literature summary extracted from

  • Berdis, A.J.; Lee, I.; Coward, J.K.; Stephens, C.; Wright, R.; Shapiro, L.; Benkovic, S.J.
    A cell cycle-regulated adenine DNA methyltransferase from Caulobacter crescentus processively methylates GANTC sites on hemimethylated DNA (1998), Proc. Natl. Acad. Sci. USA, 95, 2874-2879.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 subcloned into pET21b overexpression in Escherichia coli Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Caulobacter vibrioides the enzyme is involved in cell cycle regulation of Caulobacter crescentus S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Caulobacter vibrioides
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-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.72
-
 Caulobacter vibrioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme preferentially binds DNA before S-adenosylmethionine. S-Adenosylhomocysteine is preferentially released from the enzyme before fully methylated DNA. Binding of both substrates and methylation occurs first in a rapid step followed by regeneration of enzyme in a second rate-limiting step Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
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 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine the enzyme is involved in cell cycle regulation of Caulobacter crescentus Caulobacter vibrioides S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.72 4
-
 fully active Caulobacter vibrioides
2.1.1.72 30
-
 thermally inactivated within 20 min Caulobacter vibrioides