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Literature summary extracted from

  • Thompson, H.J.; Sharma, S.K.; Brown, S.A.
    O-Methyltransferases of furanocoumarin biosynthesis (1978), Arch. Biochem. Biophys., 188, 272-281.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.70 marked loss of activity against xanthotoxol after resalting Ruta graveolens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.69 Co2+
-
 Ruta graveolens
2.1.1.69 Cu2+
-
 Ruta graveolens
2.1.1.69 Fe2+
-
 Ruta graveolens
2.1.1.69 kinetin strong inhibition Ruta graveolens
2.1.1.69 Mg2+ slightly inhibits the O-methylation of bergaptol Ruta graveolens
2.1.1.69 Ni2+
-
 Ruta graveolens
2.1.1.69 p-chloromercuribenzoic acid
-
 Ruta graveolens
2.1.1.69 Zn2+
-
 Ruta graveolens
2.1.1.70 Co2+
-
 Ruta graveolens
2.1.1.70 Cu2+
-
 Ruta graveolens
2.1.1.70 Fe2+
-
 Ruta graveolens
2.1.1.70 kinetin strong inhibition Ruta graveolens
2.1.1.70 Mg2+
-
 Ruta graveolens
2.1.1.70 Ni2+
-
 Ruta graveolens
2.1.1.70 p-chloromercuribenzoic acid
-
 Ruta graveolens
2.1.1.70 Zn2+
-
 Ruta graveolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.69 110000
-
 gel filtration Ruta graveolens
2.1.1.70 850000
-
 gel filtration Ruta graveolens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.69 S-adenosyl-L-methionine + bergaptol Ruta graveolens furanocoumarinbiosynthesis S-adenosyl-L-homocysteine + bergapten
-
 ?
2.1.1.70 S-adenosyl-L-methionine + xanthotoxol Ruta graveolens furanocoumarin biosynthesis S-adenosyl-L-homocysteine + xanthotoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.69 Heracleum maximum
-
 Michx.
-
2.1.1.69 Heracleum maximum Michx.
-
 Michx.
-
2.1.1.69 Ruta graveolens
-
-
-
2.1.1.70 Heracleum maximum
-
 Michx.
-
2.1.1.70 Heracleum maximum Michx.
-
 Michx.
-
2.1.1.70 Ruta graveolens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.69 leaf
-
 Heracleum maximum
-
2.1.1.69 leaf sterile leaf cell culture Ruta graveolens
-
2.1.1.69 shoot young shoots of field-grown plants Ruta graveolens
-
2.1.1.70 leaf
-
 Heracleum maximum
-
2.1.1.70 leaf sterile leaf cell culture Ruta graveolens
-
2.1.1.70 shoot young shoots of field-grown plants Ruta graveolens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.69 0.000029
-
 bergaptol Ruta graveolens
2.1.1.69 0.000056
-
 5-hydroxyxanthotoxin Ruta graveolens
2.1.1.70 0.0000073
-
 xanthotoxol Ruta graveolens
2.1.1.70 0.000033
-
 8-hydroxybergapten Ruta graveolens

Storage Stability

EC Number Storage Stability Organism
2.1.1.69 -20°C, stored and refrozen, activity against bergaptol is essentially unchanged after 18 days and is still 65% of the initial value after 34 days Ruta graveolens
2.1.1.69 0°C, stored below undesalted, 40% loss of activity after 5 weeks Ruta graveolens
2.1.1.70 -20°C, stored and refrozen, activity against xanthotoxol falls rapidly to less than half of the initial value in 1 week and to only 10% after 4 weeks Ruta graveolens
2.1.1.70 0°C, stored below undesalted, 40% loss of activity after 5 weeks Ruta graveolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.69 additional information activity against phenol, resorcinol, phloroglucinol, 3-hydroxy-4-methoxybenzaldehyde, 2-hydroxybenzoic acid, 3-hydroxybenzoic acid, 2-hydroxyphenylacetic acid, 3-hydroxycinnamic acid, and quercetin-7,4'-dimethyl ether lower than 10% of the activity observed with bergaptol as substrate Ruta graveolens ?
-
 ?
2.1.1.69 S-adenosyl-L-methionine + 5-hydroxyxanthotoxin
-
 Ruta graveolens S-adenosyl-L-homocysteine + isopimpinellin
-
 ?
2.1.1.69 S-adenosyl-L-methionine + 5-hydroxyxanthotoxin
-
 Heracleum maximum S-adenosyl-L-homocysteine + isopimpinellin
-
 ?
2.1.1.69 S-adenosyl-L-methionine + 5-hydroxyxanthotoxin
-
 Heracleum maximum Michx. S-adenosyl-L-homocysteine + isopimpinellin
-
 ?
2.1.1.69 S-adenosyl-L-methionine + bergaptol
-
 Ruta graveolens S-adenosyl-L-homocysteine + bergapten
-
 ?
2.1.1.69 S-adenosyl-L-methionine + bergaptol
-
 Heracleum maximum S-adenosyl-L-homocysteine + bergapten
-
 ?
2.1.1.69 S-adenosyl-L-methionine + bergaptol furanocoumarinbiosynthesis Ruta graveolens S-adenosyl-L-homocysteine + bergapten
-
 ?
2.1.1.69 S-adenosyl-L-methionine + bergaptol
-
 Heracleum maximum Michx. S-adenosyl-L-homocysteine + bergapten
-
 ?
2.1.1.69 S-adenosyl-L-methionine + caffeic acid
-
 Ruta graveolens S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.70 S-adenosyl-L-methionine + 7,8-dihydroxycoumarin
-
 Ruta graveolens ?
-
 ?
2.1.1.70 S-adenosyl-L-methionine + 8-hydroxybergapten
-
 Ruta graveolens S-adenosyl-L-homocysteine + isopimpinellin
-
 ?
2.1.1.70 S-adenosyl-L-methionine + 8-hydroxybergapten
-
 Heracleum maximum S-adenosyl-L-homocysteine + isopimpinellin
-
 ?
2.1.1.70 S-adenosyl-L-methionine + 8-hydroxybergapten
-
 Heracleum maximum Michx. S-adenosyl-L-homocysteine + isopimpinellin
-
 ?
2.1.1.70 S-adenosyl-L-methionine + xanthotoxol
-
 Ruta graveolens S-adenosyl-L-homocysteine + xanthotoxin
-
 ?
2.1.1.70 S-adenosyl-L-methionine + xanthotoxol
-
 Heracleum maximum S-adenosyl-L-homocysteine + xanthotoxin
-
 ?
2.1.1.70 S-adenosyl-L-methionine + xanthotoxol furanocoumarin biosynthesis Ruta graveolens S-adenosyl-L-homocysteine + xanthotoxin
-
 ?
2.1.1.70 S-adenosyl-L-methionine + xanthotoxol
-
 Heracleum maximum Michx. S-adenosyl-L-homocysteine + xanthotoxin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.69 7.3 8
-
 Ruta graveolens
2.1.1.69 8.5 9 5-hydroxyanthotoxin as substrate Ruta graveolens
2.1.1.70 7.3 8
-
 Ruta graveolens