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Literature summary extracted from

  • Collendavelloo, J.; Legrand, M.; Geoffroy, P.; Barthelemy, J.; Fritig, B.
    Purification and properties of the three o-diphenol-O-methyltransferases of tobacco leaves (1981), Phytochemistry, 20, 611-616.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.1.1.68 2-mercaptoethanol stabilizes Nicotiana tabacum
2.1.1.68 freezing causes complete inactivation Nicotiana tabacum
2.1.1.68 gel filtration leads to severe loss of activity Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.68 0.0045
-
 S-adenosyl-L-methionine
-
 Nicotiana tabacum
2.1.1.68 0.03
-
 esculetin isozyme I, m- and p-methylation Nicotiana tabacum
2.1.1.68 0.04
-
 homocatechol isozyme I Nicotiana tabacum
2.1.1.68 0.065
-
 5-hydroxyferulic acid
-
 Nicotiana tabacum
2.1.1.68 0.1
-
 caffeic acid isozyme I Nicotiana tabacum
2.1.1.68 0.23
-
 Hydrocaffeic acid isozyme I Nicotiana tabacum
2.1.1.68 0.7
-
 Protocatechuic aldehyde isozyme I, m- and p-methylation Nicotiana tabacum
2.1.1.68 2
-
 catechol isozyme I Nicotiana tabacum
2.1.1.68 2.5
-
 chlorogenic acid isozyme I Nicotiana tabacum
2.1.1.68 2.5
-
 protocatechuic acid isozyme I, m- and p-methylation Nicotiana tabacum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.68 additional information no Mg2+ requirement Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.68 93000
-
 isozyme I, gel filtration Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate Nicotiana tabacum i.e. caffeic acid, involved in the biosynthesis of lignin S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Nicotiana tabacum
-
 tobacco, strain Samsun NN, three isozymes isozyme I, isozyme II and isozyme III, only isozyme I has the characteristics of caffeate O-methyltransferase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.68 three caffeic acid methylating activities: isozymes I, II, III, with an enzyme density of 1.277 kg/l, determined by isopycnic equilibrium centrifugation Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.68 leaf highly infected by tobacco mosaic virus Nicotiana tabacum
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.68 0°C, 65%-85% of initial activity retained after 5 months in the presence of 2-mercaptoethanol Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 additional information p- and m-methylation of protocatechuic aldehyde and acid, as well as esculetin Nicotiana tabacum ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-hydrocinnamic acid
-
 Nicotiana tabacum S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxyhydrocinnamic acid
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate i.e. caffeate, m-specific methylation Nicotiana tabacum S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate i.e. caffeic acid, involved in the biosynthesis of lignin Nicotiana tabacum S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 3-(3,4-dihydroxyphenyl)propanoic acid
-
 Nicotiana tabacum S-adenosyl-L-homocysteine + 3-(4-hydroxy-3-methoxyphenyl)propanoic acid
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyferulic acid best substrate Nicotiana tabacum S-adenosyl-L-homocysteine + sinapic acid
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyferulic acid m-specific methylation Nicotiana tabacum S-adenosyl-L-homocysteine + sinapic acid
-
 ?
2.1.1.68 S-adenosyl-L-methionine + catechol isozyme II and III, no substrate for isozyme I Nicotiana tabacum S-adenosyl-L-homocysteine + 2-methoxyphenol
-
 ?
2.1.1.68 S-adenosyl-L-methionine + chlorogenic acid
-
 Nicotiana tabacum ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + esculetin
-
 Nicotiana tabacum ?
-
 ?
2.1.1.68 S-adenosyl-L-methionine + homocatechol
-
 Nicotiana tabacum S-adenosyl-L-homocysteine + 2-methoxy-4-methylphenol
-
 ?
2.1.1.68 S-adenosyl-L-methionine + protocatechuic acid
-
 Nicotiana tabacum S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzoate
-
 ?
2.1.1.68 S-adenosyl-L-methionine + protocatechuic aldehyde
-
 Nicotiana tabacum S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxybenzaldehyde
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.68 45
-
 isozyme I inactivates after 5 min, isozyme II loses 70% of activity after 2 h, isozyme III has 50% activation after 2 h Nicotiana tabacum