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Literature summary extracted from

  • Woodson, L.C.; Weinshilboum, R.M.
    Human kidney thiopurine methyltransferase. Purification and biochemical properties (1983), Biochem. Pharmacol., 32, 819-826.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.67 SKF-525A
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.67 6-methylmercaptopurine
-
 Homo sapiens
2.1.1.67 Ethacrynic acid
-
 Homo sapiens
2.1.1.67 N-ethylmaleimide
-
 Homo sapiens
2.1.1.67 S-adenosyl-L-homocysteine
-
 Homo sapiens
2.1.1.67 tropolone
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.67 0.0027
-
 S-adenosyl-L-methionine
-
 Homo sapiens
2.1.1.67 0.3
-
 6-Mercaptopurine
-
 Homo sapiens
2.1.1.67 0.55
-
 6-thioguanine
-
 Homo sapiens
2.1.1.67 2
-
 2-Thiouracil
-
 Homo sapiens
2.1.1.67 168
-
 2-mercaptoethanol
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.67 Mg2+ baseline activity 102% Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.67 36000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.67 S-adenosyl-L-methionine + a thiopurine Homo sapiens S-methylation, enzyme of major catabolic pathway of thiopurines S-adenosyl-L-homocysteine + a thiopurine S-methylether
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.67 Homo sapiens
-
 human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.67 partially Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.67 blood
-
 Homo sapiens
-
2.1.1.67 erythrocyte
-
 Homo sapiens
-
2.1.1.67 kidney
-
 Homo sapiens
-
2.1.1.67 lymphocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.67 1.03
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.67 additional information aliphatic thiol compounds are poor substrates or not methylated, dithiothreitol is no substrate Homo sapiens ?
-
 ?
2.1.1.67 S-adenosyl-L-methionine + 2-mercaptoethanol
-
 Homo sapiens S-adenosyl-L-homocysteine + 2-methylmercaptoethanol
-
 ?
2.1.1.67 S-adenosyl-L-methionine + 2-thiouracil
-
 Homo sapiens S-adenosyl-L-homocysteine + thiouracil-2-S-methylether
-
 ?
2.1.1.67 S-adenosyl-L-methionine + 6-mercaptopurine
-
 Homo sapiens S-adenosyl-L-homocysteine + 6-methylmercaptopurine
-
 ?
2.1.1.67 S-adenosyl-L-methionine + 6-thioguanine
-
 Homo sapiens S-adenosyl-L-homocysteine + 6-methylthioguanine
-
 ?
2.1.1.67 S-adenosyl-L-methionine + 6-thiopurine
-
 Homo sapiens S-adenosyl-L-homocysteine + 6-methylthiopurine
-
 ?
2.1.1.67 S-adenosyl-L-methionine + a thiopurine
-
 Homo sapiens S-adenosyl-L-homocysteine + a thiopurine S-methylether
-
 ?
2.1.1.67 S-adenosyl-L-methionine + a thiopurine S-methylation, enzyme of major catabolic pathway of thiopurines Homo sapiens S-adenosyl-L-homocysteine + a thiopurine S-methylether
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.67 6.7
-
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.67 0.00075
-
 S-adenosyl-L-homocysteine
-
 Homo sapiens
2.1.1.67 0.85
-
 tropolone
-
 Homo sapiens