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Literature summary extracted from

  • Ayabe, S.; Yoshikawa, T.; Kobayashi, M.; Furuya, T.
    Biosynthesis of a retrochalcone echinatin: involvement of o-methyltransferase to licodione (1980), Phytochemistry, 19, 2331-2336.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.65 EDTA slight increase of activity Glycyrrhiza echinata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.65 Cu2+ 80% loss of activity Glycyrrhiza echinata
2.1.1.65 Zn2+ 30% loss of activity Glycyrrhiza echinata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.65 0.0028
-
 S-adenosyl-L-methionine
-
 Glycyrrhiza echinata
2.1.1.65 0.011
-
 licodione
-
 Glycyrrhiza echinata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.65 Mg2+ not requirement Glycyrrhiza echinata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.65 S-adenosyl-L-methionine + licodione Glycyrrhiza echinata involved in retrochalcone echinatin biosynthesis, selective induction in response to stress S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.65 Glycyrrhiza echinata
-
 strains W, KN, MS, DK
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.65
-
 Glycyrrhiza echinata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.65 cell culture
-
 Glycyrrhiza echinata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.65 additional information
-
-
 Glycyrrhiza echinata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.65 S-adenosyl-L-methionine + 2'-deoxylicodione very poor substrate Glycyrrhiza echinata S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.65 S-adenosyl-L-methionine + 2'-hydroxylicodione methylated at 38% of licodione methylation Glycyrrhiza echinata S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.65 S-adenosyl-L-methionine + 2,4-dihydroxybenzoic acid methylated at 4.5% of licodione methylation Glycyrrhiza echinata S-adenosyl-L-homocysteine + 2-methoxy-4-hydroxybenzoic acid
-
 ?
2.1.1.65 S-adenosyl-L-methionine + dihydroisoliquiritigenin methylated at 7.2% of licodione methylation Glycyrrhiza echinata S-adenosyl-L-homocysteine + 2'-O-methyldihydroisoliquiritigenin
-
 ?
2.1.1.65 S-adenosyl-L-methionine + echinatin methylated at 4% of licodione methylation Glycyrrhiza echinata S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.65 S-adenosyl-L-methionine + isoliquiritigenin methylated at 10.7% of licodione methylation Glycyrrhiza echinata S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
-
 ?
2.1.1.65 S-adenosyl-L-methionine + licodione i.e. 1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)-1,3-propanedione Glycyrrhiza echinata S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
 ?
2.1.1.65 S-adenosyl-L-methionine + licodione hydroxy-group ortho to C3-linkage in licodione is methylated Glycyrrhiza echinata S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
 ?
2.1.1.65 S-adenosyl-L-methionine + licodione involved in retrochalcone echinatin biosynthesis, selective induction in response to stress Glycyrrhiza echinata S-adenosyl-L-homocysteine + 2'-O-methyllicodione
-
 ?
2.1.1.65 S-adenosyl-L-methionine + resacetophenone methylated at 2.6% of licodione methylation Glycyrrhiza echinata S-adenosyl-L-homocysteine + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.65 30
-
 assay at Glycyrrhiza echinata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.65 8
-
-
 Glycyrrhiza echinata