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Literature summary extracted from

  • Kreklau, E.L.; Liu, N.; Li, Z.; Cornetta, K.; Erickson, L.C.
    Comparison of single- versus double-bolus treatments of O6-benzylguanine for depletion of O6-methylguanine DNA methyltransferase (MGMT) activity in vivo: development of a novel fluorometric oligonucleotide assay for measurement of MGMT activity (2001), J. Pharmacol. Exp. Ther., 297, 524-530.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.63 medicine the efficiacy of 6-O-benzylguanine as a chemomodulator depends on the extent of depletion of 6-O-methylguanine DNA methyltransferase in normal tissues and the optimal therapeutic index for combination of 6-O-benzylguanine and 1,3-bis(2-chloroethyl)-1-nitrosourea therapy should be achieved by depleting 6-O-methylguanine DNA methyltransferase in the target tumor for 24 h with minimal depletion in normal tissues Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.63 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.63 bone marrow
-
 Homo sapiens
-
2.1.1.63 brain
-
 Homo sapiens
-
2.1.1.63 kidney
-
 Homo sapiens
-
2.1.1.63 liver
-
 Homo sapiens
-
2.1.1.63 lung
-
 Homo sapiens
-
2.1.1.63 spleen
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.63 additional information
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 developmemt of a novel fluorometric oligonucleotide assay to measure enzyme activity in cellular and tissue extracts Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine
-
 Homo sapiens DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?