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Literature summary extracted from

  • Roy, R.; Shiota, S.; Kennel, S.J.; Raha, R.; von Wronski, M.; Brent, T.P.; Mitra, S.
    A comparative study of the biochemical properties of human and mouse recombinant O6-methylguanine-DNA methyltransferases (1995), Carcinogenesis, 16, 405-411.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.63 DNA
-
 Homo sapiens
2.1.1.63 DNA
-
 Mus musculus
2.1.1.63 Na2SO4
-
 Homo sapiens
2.1.1.63 Na2SO4
-
 Mus musculus
2.1.1.63 Na3 citrate
-
 Homo sapiens
2.1.1.63 Na3 citrate
-
 Mus musculus
2.1.1.63 NaCl 0.2 M, 80% inhibition Homo sapiens
2.1.1.63 NaCl 0.2 M, wild-type and mutant enzyme, 55% inhibition Mus musculus
2.1.1.63 RNA
-
 Homo sapiens
2.1.1.63 RNA
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.63 20900
-
 x * 20900, SDS-PAGE Homo sapiens
2.1.1.63 22500
-
 x * 22500, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine Mus musculus the enzyme repairs mutagenic and carcinogenic 6-O-alkylguanine in DNA DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine Homo sapiens the enzyme repairs mutagenic and carcinogenic 6-O-alkylguanine in DNA DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.63 Homo sapiens
-
-
-
2.1.1.63 Mus musculus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine enzyme reacts with the alkylated base in a synthetic DNA substrate poly(dC, dG, m6dG) Mus musculus DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine enzyme reacts with the alkylated base in a synthetic DNA substrate poly(dC, dG, m6dG) Homo sapiens DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine the enzyme repairs mutagenic and carcinogenic 6-O-alkylguanine in DNA Mus musculus DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?
2.1.1.63 DNA containing 6-O-methylguanine + [protein]-L-cysteine the enzyme repairs mutagenic and carcinogenic 6-O-alkylguanine in DNA Homo sapiens DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.63 ? x * 20900, SDS-PAGE Homo sapiens
2.1.1.63 ? x * 22500, SDS-PAGE Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.63 50
-
 20 min, 55-65% loss of activity in absence of DNA, 25-35% loss of activity in presence of DNA, 0.2 mg/ml Mus musculus
2.1.1.63 50
-
 20 min, 55-65% loss of activity in absence of DNA, 25-35% loss of activity in presence of DNA, 0.2 mg/ml Homo sapiens