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Literature summary extracted from

  • Paik, W.K.; DiMaria, P.
    Enzymatic methylation and demethylation of protein-bound lysine residues (1984), Methods Enzymol., 106, 274-287.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.3.4
-
 Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.4 2,6-dichlorophenolindophenol inhibitory toward the partially purified enzyme Rattus norvegicus
1.5.3.4 Co2+ inhibitory at 1.2 mM concentration Rattus norvegicus
1.5.3.4 KCN 1.3 mM, about 40% inhibition Rattus norvegicus
1.5.3.4 Ni2+ inhibitory at 1.2 mM concentration Rattus norvegicus
1.5.3.4 Zn2+ inhibitory at 1.2 mM concentration Rattus norvegicus
2.1.1.59 S-adenosyl-L-homocysteine competitive versus S-adenosyl-L-methionine Neurospora crassa
2.1.1.59 S-adenosyl-L-methionine
-
 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.4 1.05
-
 N6-methyl-L-lysine
-
 Rattus norvegicus
2.1.1.59 0.007
-
 CNBr peptide residue 1-80 of horse heart cytochrome c Neurospora crassa
2.1.1.59 0.03
-
 [cytochrome c]-L-lysine of horse heart, heme-free Neurospora crassa
2.1.1.59 0.04
-
 CNBr peptide residue 1-65 or residue 66-104 of horse heart cytochrome c Neurospora crassa
2.1.1.59 0.2
-
 [cytochrome c]-L-lysine of horse heart, ethanol-denatured Neurospora crassa
2.1.1.59 0.32
-
 [cytochrome c]-L-lysine of horse heart, native Neurospora crassa
2.1.1.59 1.7
-
 [cytochrome c]-L-lysine
-
 Neurospora crassa
2.1.1.59 19
-
 S-adenosyl-L-methionine
-
 Neurospora crassa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.59 cytosol
-
 Neurospora crassa 5829
-
2.1.1.59 additional information overview Neurospora crassa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.59 120000
-
 gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.4 epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O Rattus norvegicus methylation of lysyl residues is catalyzed by S-adenosylmethionine:protein (lysine) N-methyltransferase demethylated lysine residues + formaldehyde + H2O2
-
 ir
1.5.3.4 epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O Rattus norvegicus protein: e.g. histones demethylated lysine residues + formaldehyde + H2O2
-
 ir
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine Neurospora crassa
-
 S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.4 Rattus norvegicus
-
-
-
2.1.1.59 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.4 16fold partial purification Rattus norvegicus
1.5.3.4 enzyme from kidney Rattus norvegicus
2.1.1.59
-
 Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.4 kidney
-
 Rattus norvegicus
-
2.1.1.59 mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.4 0.00106
-
 N6-methyl-L-lysyl-histone Rattus norvegicus
1.5.3.4 0.0283
-
 N6-methyl-L-lysine Rattus norvegicus
1.5.3.4 0.0433
-
 16fold purified enzyme Rattus norvegicus
2.1.1.59 0.0295
-
 purified enzyme Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
2.1.1.59 0-4°C, 50% saturated ammonium sulfate, stable for several months Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.4 6-(methylamino)-2-oxohexanoic acid + H2O + O2
-
 Rattus norvegicus 6-amino-2-oxohexanoic acid + formaldehyde + H2O2
-
 ?
1.5.3.4 epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O methylation of lysyl residues is catalyzed by S-adenosylmethionine:protein (lysine) N-methyltransferase Rattus norvegicus demethylated lysine residues + formaldehyde + H2O2
-
 ir
1.5.3.4 epsilon-N-methyl groups in protein-bound methyllysine residues + O2 + H2O protein: e.g. histones Rattus norvegicus demethylated lysine residues + formaldehyde + H2O2
-
 ir
1.5.3.4 additional information not: delta-N-monomethyl-L-ornithine Rattus norvegicus ?
-
 ?
1.5.3.4 N6,N6-dimethyl-L-lysine + O2 + H2O
-
 Rattus norvegicus L-lysine + formaldehyde + H2O2
-
 ?
1.5.3.4 N6-methyl-L-lysine + O2 + H2O
-
 Rattus norvegicus L-lysine + formaldehyde + H2O2
-
 ir
1.5.3.4 N6-methyl-L-lysyl-histone + O2 + H2O
-
 Rattus norvegicus histone + formaldehyde + H2O2
-
 ?
2.1.1.59 S-adenosyl-L-methionine + CNBr peptide
-
 Neurospora crassa ?
-
 ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine
-
 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine
-
 ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine horse heart cytochrome c-72 Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine also CNBr peptides of horse heart cytochrome c can serve as substrates, overview Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine a single lysine residue at position 72 of horse heart cytochrome c is methylated ?
2.1.1.59 S-adenosyl-L-methionine + cytochrome c L-lysine also CNBr peptides of horse heart cytochrome c can serve as substrates, overview Neurospora crassa S-adenosyl-L-homocysteine + cytochrome c N6-methyl-L-lysine mixture of epsilon-N-monomethyllysine, epsilon-N-dimethyllysine and epsilon-N-trimethyllysine in the ratio 1:3:4 ?
2.1.1.59 S-adenosyl-L-methionine + [cytochrome c]-L-lysine
-
 Neurospora crassa S-adenosyl-L-homocysteine + [cytochrome c]-N6-methyl-L-lysine
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.5.3.4 More same enzyme is responsible for the demethylation of epsilon-N-methyl-L-lysine and of methylated histone Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.59 37
-
 assay at Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.4 7.2
-
 phosphate buffer Rattus norvegicus
2.1.1.59 9
-
 assay at Neurospora crassa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.59 0.002
-
 S-adenosyl-L-methionine
-
 Neurospora crassa