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Literature summary extracted from

  • Locht, C.; Beaudart, J.L.; Delcour, J.
    Partial purification and characterization of mRNA (guanine-7-) methyltransferase from the yeast Saccharomyces cerevisiae (1983), Eur. J. Biochem., 134, 117-121.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.56 Ca2+ strong Saccharomyces cerevisiae
2.1.1.56 Mg2+ strong Saccharomyces cerevisiae
2.1.1.56 Mn2+ strong Saccharomyces cerevisiae
2.1.1.56 additional information inhibition by a natural low-molecular-weight inhibitor in the crude extract, removed during purification Saccharomyces cerevisiae
2.1.1.56 S-adenosyl-L-homocysteine product inhibition, competitive Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.56 0.002
-
 S-adenosyl-L-methionine
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.56 KCl no activation Saccharomyces cerevisiae
2.1.1.56 Mg2+ inactivation Saccharomyces cerevisiae
2.1.1.56 Mn2+ inhibition Saccharomyces cerevisiae
2.1.1.56 additional information no activation by NaCl Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.56 49000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.56 Saccharomyces cerevisiae
-
 osmotic sensitive
-
2.1.1.56 Saccharomyces cerevisiae VY1160
-
 osmotic sensitive
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.56
-
 Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.56 cell suspension culture
-
 Saccharomyces cerevisiae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.56 0.00000235
-
 purified enzyme Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.1.1.56 -80°C, several months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA methylates also undermethylated poly(A)-rich mRNA Saccharomyces cerevisiae S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA methylates also undermethylated poly(A)-rich mRNA Saccharomyces cerevisiae VY1160 S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?

Subunits

EC Number Subunits Comment Organism
2.1.1.56 monomer 1 * 49000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.56 30
-
 around Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.56 7
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.56 5 8 half-maximal activity at pH 5.0, unstable above pH 8.0 Saccharomyces cerevisiae