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Literature summary extracted from

  • Ensinger, M.J.; Moss, B.
    Modification of the 5 terminus of mRNA by an RNA (guanine-7-)-methyltransferase from HeLa cells (1976), J. Biol. Chem., 251, 5283-5291.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.56 cytoplasm 70% of total activity, 1/3 of which is losely associated to ribosomes Homo sapiens 5737
-
2.1.1.56 nucleus 30% of total activity Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.56 KCl
-
 Homo sapiens
2.1.1.56 Mg2+ activation Homo sapiens
2.1.1.56 additional information
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.56 56000
-
 gel filtration, sucrose density gradient centrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.56 Homo sapiens
-
 HeLa cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.56 DNA-agarose affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.56 cell suspension culture
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.56 0.000593
-
 purified enzyme Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.1.1.56 -80°C, several months Homo sapiens
2.1.1.56 4°C, t1/2: less than 36 h Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.56 additional information no substrates are poly-(A) terminating in a single phosphate or a diphosphate Homo sapiens ?
-
 ?
2.1.1.56 additional information GDP, GTP, G(5')ppppG Homo sapiens ?
-
 ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppN N: G Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppG
-
 ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA mRNA, synthetic polyribonucleotides terminating in a dinucleoside triphosphate Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA specific for mRNAs with 5'-terminal sequence G(5')pppA- and G(5')pppG- Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA R may be guanosine or adenosine, methyl acceptors are unmethylated vaccinia virus mRNAs Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA active also on E. coli tRNA Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA free G(5')pppG is utilized, G(5')ppG is a poor substrate, while GTP, GDP, and G(5')ppppG are no substrates Homo sapiens S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.56 7.2 7.8
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.56 additional information no requirement of GTP Homo sapiens