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Literature summary extracted from

  • Cong, P.; Shuman, S.
    Methyltransferase and subunit association domains of vaccinia virus mRNA capping enzyme (1992), J. Biol. Chem., 267, 16424-16429.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.56 2 carboxyl-truncated versions of the large subunit of the mRNA capping enzyme, expression in Escherichia coli Vaccinia virus
2.1.1.56 vaccinia virus genes D1 and D12 encoding the large and the small subunit of the mRNA capping enzyme, respectively, individual overexpression in Escherichia coli BL21 Vaccinia virus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.56 additional information construction of carboxyl-truncated D1 genes, amino acid 1-666 and 1-425: 347 residues are sufficient for activity Vaccinia virus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.56 S-(3-deazaadenosyl)-L-homocysteine
-
 Vaccinia virus
2.1.1.56 S-(N6-Methyladenosyl)-L-homocysteine
-
 Vaccinia virus
2.1.1.56 S-Adenosyl-D-homocysteine
-
 Vaccinia virus
2.1.1.56 S-adenosyl-L-cysteine
-
 Vaccinia virus
2.1.1.56 S-adenosyl-L-homocysteine
-
 Vaccinia virus
2.1.1.56 S-Adenosyl-L-homocysteine sulfone
-
 Vaccinia virus
2.1.1.56 S-adenosyl-L-homocysteine sulfoxide
-
 Vaccinia virus
2.1.1.56 S-Aristeromycinyl-L-homocysteine
-
 Vaccinia virus
2.1.1.56 S-Tubercidinyl-L-homocysteine
-
 Vaccinia virus
2.1.1.56 sinefungin
-
 Vaccinia virus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.56 viral nucleocapsid
-
 Vaccinia virus 19013
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.56 additional information
-
 mutants Vaccinia virus
2.1.1.56 31000
-
 1 * 31000 + 1 * 95000, SDS-PAGE Vaccinia virus
2.1.1.56 95000
-
 1 * 31000 + 1 * 95000, SDS-PAGE Vaccinia virus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA Vaccinia virus the presence of the small one is required for methylase activity, the large subunit bears functional domains for binding of the small subunit and for methylase activity S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA Vaccinia virus one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.56 Vaccinia virus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.56 S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-N7-)methyltransferase form the mRNA capping enzyme complex consiting of a small and a large subunit Vaccinia virus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA
-
 Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA the presence of the small one is required for methylase activity, the large subunit bears functional domains for binding of the small subunit and for methylase activity Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA one of a series of three reactions in capping of vaccinia virus mRNA catalyzed by one enzyme, which is a heterodimer: no methylase activity with one of the subunits alone Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.56 dimer 1 * 31000 + 1 * 95000, SDS-PAGE Vaccinia virus
2.1.1.56 More subunit organisation of the mRNA capping enzyme complex Vaccinia virus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.56 37
-
 assay at Vaccinia virus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.56 0.00000018
-
 amino acid modified structure analogue of adenosyl-L-homocysteine
-
 Vaccinia virus
2.1.1.56 0.0000075
-
 sinefungin
-
 Vaccinia virus
2.1.1.56 0.00053
-
 S-adenosyl-L-homocysteine
-
 Vaccinia virus
2.1.1.56 0.0012
-
 S-Adenosyl-L-homocysteine sulfone
-
 Vaccinia virus
2.1.1.56 0.0012
-
 S-adenosyl-L-homocysteine sulfoxide
-
 Vaccinia virus
2.1.1.56 0.0012
-
 S-Tubercidinyl-L-homocysteine
-
 Vaccinia virus
2.1.1.56 0.0021
-
 S-(3-deazaadenosyl)-L-homocysteine
-
 Vaccinia virus
2.1.1.56 0.0026
-
 S-Aristeromycinyl-L-homocysteine
-
 Vaccinia virus
2.1.1.56 0.0046
-
 S-(3'-aminoadenosyl)-L-homocysteine
-
 Vaccinia virus
2.1.1.56 0.009
-
 S-(N6-Methyladenosyl)-L-homocysteine
-
 Vaccinia virus
2.1.1.56 0.014
-
 S-Adenosyl-D-homocysteine
-
 Vaccinia virus
2.1.1.56 0.058
-
 S-adenosyl-L-cysteine
-
 Vaccinia virus