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Literature summary extracted from

  • Pugh, C.S.G.; Borchardt, R.T.
    Effects of S-adenosylhomocysteine analogues on vaccinia viral messenger ribonucleic acid synthesis and methylation (1982), Biochemistry, 21, 1535-1541.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.56 5',N8-Adenosyl-alpha,beta-diaminobutyric acid moderate Vaccinia virus
2.1.1.56 amino acid modified structure analogue of adenosyl-L-homocysteine i.e. A9145C, strong Vaccinia virus
2.1.1.56 additional information no inhibition by S-uridinyl-L-homocysteine, S-cytidinyl-L-homocysteine Vaccinia virus
2.1.1.56 S-(2-azaadenosyl)-L-homocysteine moderate Vaccinia virus
2.1.1.56 S-(3-Aminoadenosyl)-L-homocysteine weak Vaccinia virus
2.1.1.56 S-(3-deazaadenosyl)-L-homocysteine strong Vaccinia virus
2.1.1.56 S-(8-azaadenosyl)-L-homocysteine weak Vaccinia virus
2.1.1.56 S-(N6-Dimethyl-3-deazaadenosyl)-L-homocysteine weak Vaccinia virus
2.1.1.56 S-(N6-Methyladenosyl)-L-homocysteine moderate Vaccinia virus
2.1.1.56 S-Adenosyl-D-homocysteine weak Vaccinia virus
2.1.1.56 S-adenosyl-L-cysteine moderate Vaccinia virus
2.1.1.56 S-adenosyl-L-homocysteine product inhibition, competitive Vaccinia virus
2.1.1.56 S-Adenosyl-L-homocysteine structural analogues
-
 Vaccinia virus
2.1.1.56 S-Adenosyl-L-homocysteine sulfone weak Vaccinia virus
2.1.1.56 S-adenosyl-L-homocysteine sulfoxide weak Vaccinia virus
2.1.1.56 S-Aristeromycinyl-L-homocysteine
-
 Vaccinia virus
2.1.1.56 S-Inosyl-L-homocysteine weak Vaccinia virus
2.1.1.56 S-Tubercidinyl-L-homocysteine weak Vaccinia virus
2.1.1.56 sinefungin i.e. A9145, strong Vaccinia virus
2.1.1.57 5',N8-Adenosyl-alpha,beta-diaminobutyric acid moderate Vaccinia virus
2.1.1.57 A9145C strong Vaccinia virus
2.1.1.57 S-(2-azaadenosyl)-L-homocysteine
-
 Vaccinia virus
2.1.1.57 S-(3'-aminoadenosyl)-L-homocysteine
-
 Vaccinia virus
2.1.1.57 S-(3-deazaadenosyl)-L-homocysteine strong Vaccinia virus
2.1.1.57 S-(8-azaadenosyl)-L-homocysteine weak Vaccinia virus
2.1.1.57 S-(N6-Dimethyl-3-deazaadenosyl)-L-homocysteine weak Vaccinia virus
2.1.1.57 S-(N6-Methyladenosyl)-L-homocysteine
-
 Vaccinia virus
2.1.1.57 S-Adenosyl-D-homocysteine
-
 Vaccinia virus
2.1.1.57 S-adenosyl-L-cysteine moderate Vaccinia virus
2.1.1.57 S-adenosyl-L-homocysteine strong Vaccinia virus
2.1.1.57 S-Adenosyl-L-homocysteine sulfone strong Vaccinia virus
2.1.1.57 S-adenosyl-L-homocysteine sulfoxide strong Vaccinia virus
2.1.1.57 S-Aristeromycinyl-L-homocysteine
-
 Vaccinia virus
2.1.1.57 S-Cytidyl-L-homocysteine weak Vaccinia virus
2.1.1.57 S-Inosyl-L-homocysteine weak Vaccinia virus
2.1.1.57 S-Tubercidinyl-L-homocysteine strong Vaccinia virus
2.1.1.57 S-Uridyl-L-homocysteine weak Vaccinia virus
2.1.1.57 sinefungin strong Vaccinia virus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.56 viral nucleocapsid
-
 Vaccinia virus 19013
-
2.1.1.57 viral nucleocapsid
-
 Vaccinia virus 19013
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA Vaccinia virus R may be guanosine or adenosine, involved in synthesis of methylated capped structure at 5'-terminal of viral mRNA S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA Vaccinia virus WR R may be guanosine or adenosine, involved in synthesis of methylated capped structure at 5'-terminal of viral mRNA S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?
2.1.1.57 S-adenosyl-L-methionine + m7G(5')pppR-RNA Vaccinia virus final step in formation of 7-methylguanosine(5')pppNm-cap structure of viral RNA S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
 ?
2.1.1.57 S-adenosyl-L-methionine + m7G(5')pppR-RNA Vaccinia virus WR final step in formation of 7-methylguanosine(5')pppNm-cap structure of viral RNA S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.56 Vaccinia virus
-
-
-
2.1.1.57 Vaccinia virus
-
-
-
2.1.1.57 Vaccinia virus WR
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA
-
 Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA R may be guanosine or adenosine, involved in synthesis of methylated capped structure at 5'-terminal of viral mRNA Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA
-
 Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA R may be guanosine or adenosine, mRNA containing an N7-methylguanine cap ?
2.1.1.56 S-adenosyl-L-methionine + G(5')pppR-RNA R may be guanosine or adenosine, involved in synthesis of methylated capped structure at 5'-terminal of viral mRNA Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppR-RNA
-
 ?
2.1.1.57 S-adenosyl-L-methionine + m7G(5')pppR-RNA
-
 Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
 ?
2.1.1.57 S-adenosyl-L-methionine + m7G(5')pppR-RNA final step in formation of 7-methylguanosine(5')pppNm-cap structure of viral RNA Vaccinia virus S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
 ?
2.1.1.57 S-adenosyl-L-methionine + m7G(5')pppR-RNA
-
 Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
 ?
2.1.1.57 S-adenosyl-L-methionine + m7G(5')pppR-RNA final step in formation of 7-methylguanosine(5')pppNm-cap structure of viral RNA Vaccinia virus WR S-adenosyl-L-homocysteine + m7G(5')pppRm-RNA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.56 37
-
 assay at Vaccinia virus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.56 7.8
-
 assay at Vaccinia virus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.57 0.00000018
-
 A9145C
-
 Vaccinia virus
2.1.1.57 0.0000075
-
 sinefungin
-
 Vaccinia virus
2.1.1.57 0.00053
-
 S-adenosyl-L-homocysteine
-
 Vaccinia virus
2.1.1.57 0.0012
-
 S-Adenosyl-L-homocysteine sulfone
-
 Vaccinia virus
2.1.1.57 0.0012
-
 S-adenosyl-L-homocysteine sulfoxide
-
 Vaccinia virus
2.1.1.57 0.0012
-
 S-Tubercidinyl-L-homocysteine
-
 Vaccinia virus
2.1.1.57 0.0021
-
 S-(3-deazaadenosyl)-L-homocysteine
-
 Vaccinia virus
2.1.1.57 0.0026
-
 S-Aristeromycinyl-L-homocysteine
-
 Vaccinia virus
2.1.1.57 0.0046
-
 S-(3'-aminoadenosyl)-L-homocysteine
-
 Vaccinia virus
2.1.1.57 0.009
-
 S-(N6-Methyladenosyl)-L-homocysteine
-
 Vaccinia virus
2.1.1.57 0.014
-
 S-Adenosyl-D-homocysteine
-
 Vaccinia virus
2.1.1.57 0.058
-
 S-adenosyl-L-cysteine
-
 Vaccinia virus