EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.45 | overexpression of the encoded gene by transforming Escherichia coli Chi2913 | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.1.1.45 | - |
Escherichia coli |
2.1.1.45 | - |
Lacticaseibacillus casei |
2.1.1.45 | - |
Pneumocystis carinii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | ethylene glycol | - |
Escherichia coli | |
2.1.1.45 | glycerol | - |
Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | Mg2+ | - |
Lacticaseibacillus casei | |
2.1.1.45 | Mg2+ | high requirement | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Escherichia coli | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Pneumocystis carinii | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | dihydrofolate + dTMP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.45 | Escherichia coli | - |
- |
- |
2.1.1.45 | Lacticaseibacillus casei | - |
- |
- |
2.1.1.45 | Pneumocystis carinii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.45 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Escherichia coli | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Pneumocystis carinii | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Escherichia coli | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism | Pneumocystis carinii | dihydrofolate + dTMP | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.45 | dimer | - |
Escherichia coli |
2.1.1.45 | dimer | - |
Lacticaseibacillus casei |
2.1.1.45 | dimer | - |
Pneumocystis carinii |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 4 | 40 | - |
Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 40 | - |
TS activity at 40°C falls rapidly with time | Escherichia coli |