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Literature summary extracted from

  • Saxl, R.L.; Changchien, L.M.; Hardy, L.W.; Maley, F.
    Parameters affecting the restoration of activity to inactive mutants of thymidylate synthase via subunit exchange: Further evidence that thymidylate synthase Is a half-of-the-sites activity enzyme (2001), Biochemistry, 40, 5275-5282.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 overexpression of the encoded gene by transforming Escherichia coli Chi2913 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.45
-
Escherichia coli
2.1.1.45
-
Lacticaseibacillus casei
2.1.1.45
-
Pneumocystis carinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 ethylene glycol
-
Escherichia coli
2.1.1.45 glycerol
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.45 Mg2+
-
Lacticaseibacillus casei
2.1.1.45 Mg2+ high requirement Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Pneumocystis carinii biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Pneumocystis carinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Pneumocystis carinii dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Escherichia coli dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Pneumocystis carinii dihydrofolate + dTMP
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
Escherichia coli
2.1.1.45 dimer
-
Lacticaseibacillus casei
2.1.1.45 dimer
-
Pneumocystis carinii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.45 4 40
-
Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.45 40
-
TS activity at 40°C falls rapidly with time Escherichia coli