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Literature summary extracted from

  • Mahdavian, E.; Spencer, H.T.; Dunlap, R.B.
    Kinetic studies on drug-resistant variants of Escherichia coli thymidylate sythase: Functional effects of amino acid sunstitutions at residue 4 (1999), Arch. Biochem. Biophys., 368, 257-264.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.45 medicine enzyme is believed to be the major site of the cytotoxic action of the cancer chemotherapeutic drug 5-fluorouracil, development of inhibitors as clinical useful drugs Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45
-
 Escherichia coli
2.1.1.45
-
 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.45
-
 Escherichia coli
2.1.1.45
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.0011
-
 2'-deoxyuridylate wild-type Escherichia coli
2.1.1.45 0.0012
-
 2'-deoxyuridylate mutant Y4F Escherichia coli
2.1.1.45 0.0014
-
 2'-deoxyuridylate mutant Y4I, Y4V Escherichia coli
2.1.1.45 0.003
-
 2'-deoxyuridylate mutant Y4H Escherichia coli
2.1.1.45 0.008
-
 methylenetetrahydrofolate mutant Y4F Escherichia coli
2.1.1.45 0.01
-
 methylenetetrahydrofolate wild-type Escherichia coli
2.1.1.45 0.013
-
 methylenetetrahydrofolate mutant Y4H Escherichia coli
2.1.1.45 0.015
-
 methylenetetrahydrofolate mutant Y4V Escherichia coli
2.1.1.45 0.018
-
 methylenetetrahydrofolate mutant Y4I Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens key function in DNA synthesis, repair and cell division dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Homo sapiens
-
 human
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP
-
 Escherichia coli 7,8-dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP key function in DNA synthesis, repair and cell division Homo sapiens dihydrofolate + dTMP
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.45 N5,N10-methylenetetrahydrofolate
-
 Homo sapiens