Literature summary extracted from

Anderson, A.C.; O'Neil, R.H.; DeLano, W.L.; Stroud, R.M.
The structural mechanism for half-the-sites reactivity in an enzyme, thymidylate synthase, involves a relay of changes between subunits (1999), Biochemistry, 38, 13829-13836.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.1.1.45	crystals belong to space group P2(1)2(1)2(1), a = 54.05 A, b = 66.16 A and c = 178.76 A	Pneumocystis carinii

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.1.1.45	CB3717	-	Pneumocystis carinii

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.1.1.45	5,10-methylenetetrahydrofolate + dUMP	Pneumocystis carinii	biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism	dihydrofolate + dTMP	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.1.1.45	Pneumocystis carinii	P13100	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.1.1.45	-	Pneumocystis carinii

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.1.1.45	5,10-methylenetetrahydrofolate + dUMP	-	Pneumocystis carinii	dihydrofolate + dTMP	-	r
2.1.1.45	5,10-methylenetetrahydrofolate + dUMP	biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism	Pneumocystis carinii	dihydrofolate + dTMP	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.1.1.45	dimer	-	Pneumocystis carinii

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.1.1.45	0.00009	-	CB3717	-	Pneumocystis carinii

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Release 2023.1 (January 2023)