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Literature summary extracted from

  • Matthews, D.A.; Villafranca, J.E.; Janson, C.A.; Smith, W.W.; Welsh, K.; Freer, S.
    Stereochemical mechanism of action for thymidylate synthase based on the X-ray structure of the covalent inhibitory ternary complex with 5-fluoro-2-deoxyuridylate and 5,10-methylenetetrahydrofolate (1990), J. Mol. Biol., 214, 937-948.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 Escherichia coli transformed with a high amplifying expression plasmid containing the Escherichia coli thymidylate synthase gene Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.45 crystals of TS apoprotein grown using hanging drop method of vapor diffusion, cubic Laue group m3 with a = 133 A Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5,10-methylenetetrahydrofolate
-
 Escherichia coli
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Escherichia coli
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Lacticaseibacillus casei
2.1.1.45 5-fluorouracil
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 28000
-
 2 * 28000, crystallographic studies Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
 Lacticaseibacillus casei
2.1.1.45 dimer 2 * 28000, crystallographic studies Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.1.1.45 TS
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.45 (+)-methylenetetrahydrofolate
-
 Escherichia coli
2.1.1.45 (+)-methylenetetrahydrofolate
-
 Lacticaseibacillus casei
2.1.1.45 methenyltetrahydropteroylglutamate
-
 Escherichia coli