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Literature summary extracted from

  • Nakata, R.; Tsukamoto, I.; Miyoshi, M.; Kojo, S.
    Purification and characterization of thymidylate synthetase from rat regenerating liver (1987), Biochim. Biophys. Acta, 924, 297-302.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 2'-deoxyuridine
-
 Rattus norvegicus
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Homo sapiens
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Lacticaseibacillus casei
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Mus musculus
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Rattus norvegicus
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Saccharomyces cerevisiae
2.1.1.45 dTMP
-
 Rattus norvegicus
2.1.1.45 methopterin
-
 Homo sapiens
2.1.1.45 methopterin
-
 Rattus norvegicus
2.1.1.45 Procion blue
-
 Rattus norvegicus
2.1.1.45 UDP-glucuronate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.0068
-
 2'-deoxyuridylate
-
 Rattus norvegicus
2.1.1.45 0.065
-
 5,10-methylenetetrahydrofolate
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 35000
-
 2 * 35000, SDS-PAGE Rattus norvegicus
2.1.1.45 68000
-
 gel filtration Rattus norvegicus
2.1.1.45 68000
-
 gel filtration Saccharomyces cerevisiae
2.1.1.45 69000
-
 Ehrlich ascites carcinoma cells Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Homo sapiens
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Rattus norvegicus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Saccharomyces cerevisiae
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Rattus norvegicus Wistar
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Homo sapiens
-
 neoplastic leukemic CCRF-CEM cells
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Mus musculus
-
 Ehrlich ascites carcinoma cells
-
2.1.1.45 Rattus norvegicus
-
-
-
2.1.1.45 Rattus norvegicus Wistar
-
-
-
2.1.1.45 Saccharomyces cerevisiae
-
 bakers' yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Rattus norvegicus
2.1.1.45
-
 Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 liver
-
 Mus musculus
-
2.1.1.45 liver
-
 Homo sapiens
-
2.1.1.45 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.45 4°C, enzyme retains activity for about 3 weeks when stored with 20 mM dithiothreitol and 0.1 mM dUMP, without dithiothreitol purified enzyme is inactivated totally in 2 days, without dUMP enzyme keeps 50% activity after 1 week Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 (6S)-tetrahydropteroylglutamate + 2-deoxyuridylate
-
 Rattus norvegicus 7,8-dihydropteroylglutamate + thymidine phosphate
-
 ?
2.1.1.45 (6S)-tetrahydropteroylglutamate + 2-deoxyuridylate
-
 Rattus norvegicus Wistar 7,8-dihydropteroylglutamate + thymidine phosphate
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Saccharomyces cerevisiae dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Saccharomyces cerevisiae dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus Wistar dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Rattus norvegicus Wistar dihydrofolate + dTMP
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
 Mus musculus
2.1.1.45 dimer
-
 Homo sapiens
2.1.1.45 dimer
-
 Saccharomyces cerevisiae
2.1.1.45 dimer
-
 Lacticaseibacillus casei
2.1.1.45 dimer 2 * 35000, SDS-PAGE Rattus norvegicus
2.1.1.45 monomer 1 * 69000, Ehrlich ascites carcinoma cells Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.45 0.583
-
 2'-deoxyuridine
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 7.3 8 half-maximal activity at pH 6.3 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.45 7 9 70% maximal activity at either pH 7.0 or 9.0, half-maximal activity at pH 6.3 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.000005
-
 5-fluoro-2'-deoxyuridylic acid
-
 Rattus norvegicus
2.1.1.45 0.0001
-
 Procion blue variable substrate 2'-deoxyuridine Rattus norvegicus
2.1.1.45 0.00022
-
 Procion blue variable substrate 5,10-methylenetetrahydropteroylglutamate Rattus norvegicus
2.1.1.45 0.023
-
 methopterin
-
 Rattus norvegicus
2.1.1.45 0.07
-
 dTMP variable substrate 2'-deoxyuridine Rattus norvegicus
2.1.1.45 0.073
-
 methopterin variable substrate 2'-deoxyuridine Rattus norvegicus
2.1.1.45 0.24
-
 dTMP variable substrate 5,10-methylenetetrahydropteroylglutamate Rattus norvegicus