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Literature summary extracted from

  • Slavik, K.; Slavikova, V.
    Purification of thymidylate synthetase from enzyme-poor sources by affinity chromatography (1980), Methods Enzymol., 66, 709-723.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.45 enzyme can be kept active for about 2-3 weeks in buffer F in presence of 0.001 mM methotrexate, dialysis against dilute buffer leads to rapid irreversible inactivation as does freeze-drying or simple freezing Bos taurus
2.1.1.45 freezing and freeze-drying or dialysis causes rapid inactivation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Escherichia coli
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Lacticaseibacillus casei
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Mus musculus
2.1.1.45 tetrahydromethotrexate
-
 Bos taurus
2.1.1.45 tetrahydromethotrexate
-
 Escherichia coli
2.1.1.45 tetrahydromethotrexate
-
 Lacticaseibacillus casei
2.1.1.45 tetrahydromethotrexate
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 35000
-
 2 * 35000, SDS-PAGE Mus musculus
2.1.1.45 35000
-
 2 * 35000, SDS-PAGE Bos taurus
2.1.1.45 70000
-
-
 Mus musculus
2.1.1.45 70000
-
 gel filtration Escherichia coli
2.1.1.45 70000
-
 gel filtration Bos taurus
2.1.1.45 70000
-
 gel filtration Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Bos taurus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli B / ATCC 11303
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Bos taurus
-
 calf
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Escherichia coli B / ATCC 11303
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Mus musculus
-
 Gardner lymphosarcoma
-
2.1.1.45 Mus musculus
-
 mouse, sarcoma 180
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Lacticaseibacillus casei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 thymus
-
 Mus musculus
-
2.1.1.45 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 0.002135
-
 Gardner lymphosarcoma Mus musculus
2.1.1.45 0.003519
-
 sarcoma 180 Mus musculus
2.1.1.45 0.1024
-
-
 Bos taurus
2.1.1.45 0.6778
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.1.45 -20°C, occasionally loses activity Mus musculus
2.1.1.45 -50°C-to -20°C, activity of purified enzyme remains unchanged for 2-3 months Bos taurus
2.1.1.45 0-10°C enzyme is stable in buffer D for several weeks Escherichia coli
2.1.1.45 0-7°C, concentrated enzyme keeps its activity for 14 days Bos taurus
2.1.1.45 0°C, enzyme rapidly loses its activity, more than 90% in 2 h Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 (6R)-N5,N10-methylene-5,6,7,8-tetrahydrofolate + 2'-deoxyuridylate
-
 Lacticaseibacillus casei 7,8-dihydrofolate + 5-methyl-2'-deoxyuridylate
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bos taurus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bos taurus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
 Escherichia coli
2.1.1.45 dimer
-
 Lacticaseibacillus casei
2.1.1.45 dimer 2 * 35000, SDS-PAGE Mus musculus
2.1.1.45 dimer 2 * 35000, SDS-PAGE Bos taurus