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Literature summary extracted from

  • Haertle, T.; Wohlrab, F.; Guschlbauer, W.
    Thymidylate synthetase from Escherichia coli K12. Purification, and dependence of kinetic properties on sugar conformation and size of the 2 substituent (1979), Eur. J. Biochem., 102, 223-230.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.01
-
2'-deoxyuridylate
-
Escherichia coli
2.1.1.45 0.0104
-
2'-deoxyuridylate
-
Escherichia coli
2.1.1.45 0.014
-
5,10-methylene-5,6,7,8-tetrahydrofolate
-
Escherichia coli
2.1.1.45 0.11
-
2'-fluoro-2'-deoxyuridine 5'-phosphate
-
Escherichia coli
2.1.1.45 0.113
-
2'-fluoro-2'-deoxyuridine 5'-phosphate
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.45 Mg2+
-
Escherichia coli
2.1.1.45 Mg2+
-
Streptococcus pneumoniae
2.1.1.45 Mg2+
-
Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 32000
-
2 * 32000 Escherichia coli
2.1.1.45 32500
-
2 * 32500, SDS-PAGE Escherichia coli
2.1.1.45 63000 64000 gel filtration Escherichia coli
2.1.1.45 64000
-
-
Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Streptococcus pneumoniae
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia phage T2
-
dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli B / ATCC 11303 biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli EM 20031 K12 biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism dihydrofolate + dTMP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Escherichia coli B / ATCC 11303
-
-
-
2.1.1.45 Escherichia coli EM 20031 K12
-
-
-
2.1.1.45 Escherichia phage T2
-
-
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 1.47
-
-
Escherichia coli
2.1.1.45 1.52
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.1.45 -20°C, enzyme can be stored frozen for several months without significant loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + 2'-fluoro-2'-deoxyuridine 5'-phosphate
-
Escherichia coli 7,8-dihydrofolate + ?
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + 2'-fluoro-2'-deoxyuridine 5'-phosphate
-
Escherichia coli B / ATCC 11303 7,8-dihydrofolate + ?
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + 2'-fluoro-2'-deoxyuridine 5'-phosphate
-
Escherichia coli EM 20031 K12 7,8-dihydrofolate + ?
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Streptococcus pneumoniae dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Streptococcus pneumoniae dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Lacticaseibacillus casei dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia phage T2 dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia phage T2 dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Escherichia coli dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Escherichia coli B / ATCC 11303 dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Escherichia coli EM 20031 K12 dihydrofolate + dTMP
-
r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP biosynthesis of thymidylic acid, only de novo source of thymidine, crucial for DNA replication in every organism Escherichia coli EM 20031 K12 dihydrofolate + dTMP
-
?
2.1.1.45 additional information
-
Streptococcus pneumoniae ?
-
?
2.1.1.45 additional information
-
Lacticaseibacillus casei ?
-
?
2.1.1.45 additional information nucleotides with large substituents on the 2' position are no substrates Escherichia coli ?
-
?
2.1.1.45 additional information enzyme catalyzes the exchange of a hydrogen atom between water and position 5 of dUMP, occurrence of reactions other than a methyl-group transfer during the interaction of the enzyme with its 2 substrates Escherichia coli ?
-
?
2.1.1.45 additional information nucleotides with large substituents on the 2' position are no substrates Escherichia coli B / ATCC 11303 ?
-
?
2.1.1.45 additional information enzyme catalyzes the exchange of a hydrogen atom between water and position 5 of dUMP, occurrence of reactions other than a methyl-group transfer during the interaction of the enzyme with its 2 substrates Escherichia coli B / ATCC 11303 ?
-
?
2.1.1.45 additional information nucleotides with large substituents on the 2' position are no substrates Escherichia coli EM 20031 K12 ?
-
?
2.1.1.45 additional information enzyme catalyzes the exchange of a hydrogen atom between water and position 5 of dUMP, occurrence of reactions other than a methyl-group transfer during the interaction of the enzyme with its 2 substrates Escherichia coli EM 20031 K12 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 dimer
-
Streptococcus pneumoniae
2.1.1.45 dimer
-
Lacticaseibacillus casei
2.1.1.45 dimer
-
Escherichia phage T2
2.1.1.45 dimer 2 * 32500, SDS-PAGE Escherichia coli
2.1.1.45 dimer 2 * 32000 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 7 8
-
Escherichia coli