EC Number | General Stability | Organism |
---|---|---|
2.1.1.45 | thawing of frozen enzyme leads to irreversible precipitation of the protein and total loss of activity, no successfully lyophilization possible | Lacticaseibacillus casei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | 2-hydroxyethyl disulfide | - |
Lacticaseibacillus casei | |
2.1.1.45 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Lacticaseibacillus casei | |
2.1.1.45 | 5-fluoro-2'-deoxyuridylic acid | - |
Lacticaseibacillus casei | |
2.1.1.45 | cyanide | - |
Lacticaseibacillus casei | |
2.1.1.45 | H2O2 | - |
Lacticaseibacillus casei | |
2.1.1.45 | iodoacetamide | - |
Lacticaseibacillus casei | |
2.1.1.45 | N-ethylmaleimide | - |
Lacticaseibacillus casei | |
2.1.1.45 | p-chloromercuribenzoate | - |
Lacticaseibacillus casei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.45 | 0.0051 | - |
2'-deoxyuridylate | in presence of 10 mM Mg2+ | Lacticaseibacillus casei | |
2.1.1.45 | 0.0068 | - |
2'-deoxyuridylate | - |
Lacticaseibacillus casei | |
2.1.1.45 | 0.012 | - |
5,10-methylenetetrahydrofolate | in presence of 10 mM Mg2+ | Lacticaseibacillus casei | |
2.1.1.45 | 0.032 | - |
5,10-methylenetetrahydrofolate | - |
Lacticaseibacillus casei |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | Mg2+ | in Tris-acetate buffers 10 mM enhances enzyme activity by some 30% | Lacticaseibacillus casei |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 35000 | - |
2 * 35000, SDS-PAGE | Lacticaseibacillus casei |
2.1.1.45 | 70000 | - |
gel filtration | Lacticaseibacillus casei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | Lacticaseibacillus casei | - |
dihydrofolate + dTMP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.45 | Lacticaseibacillus casei | - |
var. rhamnosus, ATCC 7469 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.45 | - |
Lacticaseibacillus casei |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 2.5 | - |
- |
Lacticaseibacillus casei |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.1.45 | 0-5°C, enzyme is stable over a period of months in presence of 10 mM 2-mercaptoethanol in capped vessels layered with argon | Lacticaseibacillus casei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
? | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + dUMP | - |
Lacticaseibacillus casei | dihydrofolate + dTMP | - |
r | |
2.1.1.45 | 5,10-methylenetetrahydrofolate + uridine 5'-phosphate | - |
Lacticaseibacillus casei | dihydrofolate + TMP | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.45 | dimer | 2 * 35000, SDS-PAGE | Lacticaseibacillus casei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.45 | 5.9 | - |
UMP as substrate | Lacticaseibacillus casei |
2.1.1.45 | 6.5 | 6.8 | - |
Lacticaseibacillus casei |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.45 | (+)-methylenetetrahydrofolate | - |
Lacticaseibacillus casei |