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Literature summary extracted from

  • Horinishi, H.; Greenberg, D.M.
    Purification and properties of thymidylate synthase from calf thymus (1972), Biochim. Biophys. Acta, 258, 741-752.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.45 (NH4)2SO4 10 mM, relative activity 108% Bos taurus
2.1.1.45 ATP 0.1 mM, relative activity 102% Bos taurus
2.1.1.45 EDTA 5.0 mM, relative activity 103% Bos taurus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.45
-
 Lacticaseibacillus casei

General Stability

EC Number General Stability Organism
2.1.1.45 comparatively unstable, more stable in 20% sucrose solution Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 ATP
-
 Bos taurus
2.1.1.45 dTMP
-
 Bos taurus
2.1.1.45 dTMP
-
 Enterococcus faecalis
2.1.1.45 dTMP
-
 Gallus gallus
2.1.1.45 thymidine phosphate
-
 Bos taurus
2.1.1.45 thymidine phosphate
-
 Enterococcus faecalis
2.1.1.45 thymidine phosphate
-
 Escherichia coli
2.1.1.45 thymidine phosphate
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.45 0.004
-
 2'-deoxyuridylate
-
 Escherichia coli
2.1.1.45 0.0051
-
 2'-deoxyuridylate in presence of 10 mM Mg2+ Lacticaseibacillus casei
2.1.1.45 0.0057
-
 2'-deoxyuridylate
-
 Enterococcus faecalis
2.1.1.45 0.0075
-
 2'-deoxyuridylate
-
 Gallus gallus
2.1.1.45 0.009
-
 2'-deoxyuridylate
-
 Bos taurus
2.1.1.45 0.014
-
 2'-deoxyuridylate Ehrlich ascites carcinoma cells Mus musculus
2.1.1.45 0.018
-
 2'-deoxyuridylate phage infected E. coli Escherichia phage T2
2.1.1.45 0.02
-
 2'-deoxyuridylate less purified enzyme Bos taurus
2.1.1.45 0.03
-
 2'-deoxyuridylate phage infected E. coli Enterobacteria phage T6
2.1.1.45 0.037
-
 2'-deoxyuridylate Ehrlich ascites carcinoma cells Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Gallus gallus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia coli
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Bos taurus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Enterococcus faecalis
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Lacticaseibacillus casei
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Escherichia phage T2
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Enterobacteria phage T6
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Bos taurus
-
 calf
-
2.1.1.45 Enterobacteria phage T6
-
-
-
2.1.1.45 Enterococcus faecalis
-
-
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Escherichia phage T2
-
-
-
2.1.1.45 Gallus gallus
-
 chicken
-
2.1.1.45 Lacticaseibacillus casei
-
-
-
2.1.1.45 Mus musculus
-
 Ehrlich ascites carcinoma cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45
-
 Gallus gallus
2.1.1.45
-
 Bos taurus
2.1.1.45 Ehrlich ascites carcinoma cells Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 embryo
-
 Gallus gallus
-
2.1.1.45 thymus
-
 Mus musculus
-
2.1.1.45 thymus thymus gland Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.45 0.00035
-
-
 Bos taurus
2.1.1.45 54
-
-
 Enterococcus faecalis
2.1.1.45 150
-
-
 Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Gallus gallus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Gallus gallus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bos taurus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bos taurus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Enterococcus faecalis dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Enterococcus faecalis dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Lacticaseibacillus casei dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia phage T2 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia phage T2 dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Enterobacteria phage T6 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Enterobacteria phage T6 dihydrofolate + dTMP
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.45 38
-
 unstable above Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.45 6.5 6.8
-
 Lacticaseibacillus casei
2.1.1.45 6.5 6.8 phosphate buffer Bos taurus
2.1.1.45 6.6
-
-
 Bos taurus
2.1.1.45 7.5
-
-
 Gallus gallus
2.1.1.45 7.5 8
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.45 5.5 8
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.45 0.024
-
 dTMP
-
 Enterococcus faecalis
2.1.1.45 0.059
-
 dTMP
-
 Bos taurus
2.1.1.45 0.14
-
 dTMP
-
 Gallus gallus