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Literature summary extracted from

  • Ebel, J.; Hahlbrock, K.; Grisebach, H.
    Purification and properties of an o-dihydricphenol meta-O-methyltransferase from cell suspension cultures of parsley and its relation to flavonoid biosynthesis (1972), Biochim. Biophys. Acta, 269, 313-326.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.42 additional information extractable activity drastically increases by illuminating cells with white light Petroselinum crispum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.42 EDTA
-
 Petroselinum crispum
2.1.1.42 Mn2+
-
 Petroselinum crispum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.42 0.031
-
 5,7,3'-trihydroxyflavone 7-O-glucoside
-
 Petroselinum crispum
2.1.1.42 0.035
-
 quercetin
-
 Petroselinum crispum
2.1.1.42 0.046
-
 5,7,3'-trihydroxyflavone
-
 Petroselinum crispum
2.1.1.42 0.15
-
 S-adenosyl-L-methionine with luteolin as substrate Petroselinum crispum
2.1.1.42 1.2
-
 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-2,3-dihydro-4H-chromen-4-one
-
 Petroselinum crispum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.42 Mg2+ required for activity Petroselinum crispum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.42 48000
-
 gel filtration Petroselinum crispum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone Petroselinum crispum luteolin, enzyme directly related to flavone glycoside biosynthesis, biosynthesis of 3'-methoxyflavonoids S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone chrysoeriol ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.42 Petroselinum crispum
-
 parsley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.42 partial Petroselinum crispum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.42 0.000027
-
-
 Petroselinum crispum

Storage Stability

EC Number Storage Stability Organism
2.1.1.42 -20°C, 1-3 mg protein/ml in 0.02 M Tris-HCl buffer loses about 30% of its activity after 1 month Petroselinum crispum
2.1.1.42 4°C, acetone powder of fresh cells as source of the enzyme can be kept without significant loss of enzyme activity Petroselinum crispum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.42 additional information isomeric substances isoferulic acid and diosmetin, chrysoeriol, apigenin p-cumaric acid and ferulic acid are not substrates Petroselinum crispum ?
-
 ?
2.1.1.42 additional information only ortho-dihydric phenols can function as substrates and only the meta-hydroxyl group is methylated, no reaction takes place if one of the two phenolic hydroxyls is methylated or if only one hydroxyl group is present Petroselinum crispum ?
-
 ?
2.1.1.42 S-adenosyl-L-methionine + 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-2,3-dihydro-4H-chromen-4-one
-
 Petroselinum crispum ?
-
 ?
2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavanone eriodictyol Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavanone
-
 ?
2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone luteolin Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone chrysoeriol ?
2.1.1.42 S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone luteolin, enzyme directly related to flavone glycoside biosynthesis, biosynthesis of 3'-methoxyflavonoids Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone chrysoeriol ?
2.1.1.42 S-adenosyl-L-methionine + 5,7,3'-trihydroxyflavone
-
 Petroselinum crispum ?
-
 ?
2.1.1.42 S-adenosyl-L-methionine + 5,7,3'-trihydroxyflavone 7-O-glucoside luteolin 7-O-glucoside Petroselinum crispum S-adenosyl-L-homocysteine + 5,7,4'-trihydroxy-3'-methoxyflavone 7-O-glucoside chrysoeriol-7-O-glucoside ?
2.1.1.42 S-adenosyl-L-methionine + quercetin
-
 Petroselinum crispum S-adenosyl-L-homocysteine + 3'-O-methylquercetin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.42 9.6 9.8 glycine-NaOH buffer with caffeic acid and luteolin as substrates Petroselinum crispum
2.1.1.42 9.7
-
-
 Petroselinum crispum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.42 9.8
-
 at higher pH values activity rapidly declines Petroselinum crispum