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Literature summary extracted from

  • Kaneshiro, E.S.; Rosenfeld, J.A.; Basselin-Eiweida, M.; Stringer, J.R.; Keely, S.P.; Smulian, A.G.; Giner, J.L.
    The Pneumocystis carinii drug target s-adenosyl-L-methionine:Sterol C-24 methyl transferase has a unique substrate preference (2002), Mol. Microbiol., 44, 989-999.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.41 medicine target for drug development against pneumonitis caused by this pathogen based on its unique substrate preference for lanosterol and 24-methylenelanosterol Pneumocystis carinii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.41 expression in Escherichia coli, His-tagged protein Pneumocystis carinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.41 0.0106
-
 lanosterol recombinant enzyme Pneumocystis carinii
2.1.1.41 0.019
-
 24-methylenelanosterol recombinant enzyme Pneumocystis carinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.41 zymosterol + S-adenosyl-L-methionine Pneumocystis carinii
-
 fecosterol + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.41 Pneumocystis carinii
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.41 S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol substrate binding, amino acid sequence Pneumocystis carinii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.41 24-methylenelanosterol + S-adenosyl-L-methionine preferred substrate Pneumocystis carinii pneumocysterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.41 lanosterol + S-adenosyl-L-methionine preferred substrate Pneumocystis carinii 24-methylene-24,25-dihydrolanosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.41 zymosterol + S-adenosyl-L-methionine
-
 Pneumocystis carinii fecosterol + S-adenosyl-L-homocysteine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.41 37
-
 recombinant enzyme Pneumocystis carinii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.41 22 45 recombinant enzyme Pneumocystis carinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.41 7.5
-
 recombinant enzyme Pneumocystis carinii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.41 5.5 9 recombinant enzyme Pneumocystis carinii